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DKK4 Polyclonal antibody

DKK4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27080-1-AP

Synonyms

Dickkopf 4, Dickkopf related protein 4, DKK 4, DKK4, hDkk 4

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse cerebellum tissue using DKK4 Polyclonal antibody (27080-1-AP)

    WB analysis of mouse cerebellum using 27080-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 27080-1-AP (DKK4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using DKK4 Polyclonal antibody (27080-1-AP)

    IHC staining of human oesophagus using 27080-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 27080-1-AP (DKK4 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using DKK4 Polyclonal antibody (27080-1-AP)

    IHC staining of human oesophagus using 27080-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 27080-1-AP (DKK4 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inmouse cerebellum tissue
Positive IHC detected inhuman oesophagus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 27080-1-AP is DKK4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DKK4 fusion protein Ag24209
Full Name dickkopf homolog 4 (Xenopus laevis)
Calculated Molecular Weight 224 aa, 25 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC107047
Gene Symbol DKK4
Gene ID (NCBI) 27121
RRIDAB_2880746
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DKK4 antibody 27080-1-APDownload protocol
IHC protocol for DKK4 antibody 27080-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC,IF

Clin Transl Med

O-GlcNAcylated LARP1 positively regulated by circCLNS1A facilitates hepatoblastoma progression through DKK4/β-catenin signalling

Authors - Zhongqi Cui
View Article