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DIMT1L Polyclonal antibody

DIMT1L Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 15563-1-AP

Synonyms

18S rRNA dimethylase, DIM1, DIMT1, DIMT1L, HSA9761, HUSSY5

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using DIMT1L Polyclonal antibody (15563-1-AP)

    WB analysis using 15563-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15563-1-AP (DIMT1L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DIMT1L Polyclonal antibody (15563-1-AP)

    WB analysis of HeLa using 15563-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15563-1-AP (DIMT1L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using DIMT1L Polyclonal antibody (15563-1-AP)

    WB analysis of Jurkat using 15563-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15563-1-AP (DIMT1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using DIMT1L Polyclonal antibody (15563-1-AP)

    WB analysis of Raji using 15563-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 15563-1-AP (DIMT1L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, Raji cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15563-1-AP targets DIMT1L in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DIMT1L fusion protein Ag7721
Full Name DIM1 dimethyladenosine transferase 1-like (S. cerevisiae)
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC002841
Gene Symbol DIMT1L
Gene ID (NCBI) 27292
RRIDAB_2090821
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DIMT1L antibody 15563-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Kaposi's sarcoma-associated herpesvirus induces specialised ribosomes to efficiently translate viral lytic mRNAs

Authors - James C Murphy
View Article