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DHRS1 Polyclonal antibody

DHRS1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16275-1-AP

Synonyms

Short chain dehydrogenase/reductase family 19C member 1, SDR19C1, Protein SDR19C1, EC:1.1.1.-

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DHRS1 Polyclonal antibody (16275-1-AP)

    WB analysis using 16275-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16275-1-AP (DHRS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DHRS1 Polyclonal antibody (16275-1-AP)

    WB analysis using 16275-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16275-1-AP (DHRS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using DHRS1 Polyclonal antibody (16275-1-AP)

    WB analysis of human liver using 16275-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 16275-1-AP (DHRS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using DHRS1 Polyclonal antibody (16275-1-AP)

    IHC staining of human kidney using 16275-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16275-1-AP (DHRS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin tissue using DHRS1 Polyclonal antibody (16275-1-AP)

    IHC staining of human skin using 16275-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 16275-1-AP (DHRS1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using DHRS1 Polyclonal antibody (16275-1-AP)

    IHC staining of human skin using 16275-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 16275-1-AP (DHRS1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using DHRS1 Polyclonal antibody (16275-1-AP)

    IHC staining of human liver using 16275-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16275-1-AP (DHRS1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using DHRS1 Polyclonal antibody (16275-1-AP)

    IHC staining of human liver using 16275-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16275-1-AP (DHRS1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using DHRS1 Polyclonal antibody (16275-1-AP)

    IHC staining of human kidney using 16275-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16275-1-AP (DHRS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using DHRS1 Polyclonal antibody (16275-1-AP)

    IF Staining of A431 using 16275-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DHRS1 antibody (16275-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inCOLO 320 cells, human liver tissue, mouse colon tissue, mouse testis tissue
Positive IHC detected inhuman kidney tissue, human skin tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16275-1-AP targets DHRS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DHRS1 fusion protein Ag9351
Full Name dehydrogenase/reductase (SDR family) member 1
Calculated Molecular Weight 313 aa, 34 kDa
Observed Molecular Weight 35-38 kDa
GenBank Accession NumberBC014057
Gene Symbol DHRS1
Gene ID (NCBI) 115817
RRIDAB_2292847
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DHRS1(Dehydrogenase/reductase SDR family member 1) is also named as LJ25430, MGC20204, DKFZp586I0523, SDR19C1, FLJ14250 and belongs to the short-chain dehydrogenases/reductases (SDR) family. It is involved in the metabolism of a large variety of compounds, including steroid hormones, prostaglandins, retinoids, lipids and xenobiotics.Northern blot analysis detected DHRS1express in heart highest and in liver lowest.

Protocols

Product Specific Protocols
WB protocol for DHRS1 antibody 16275-1-APDownload protocol
IHC protocol for DHRS1 antibody 16275-1-APDownload protocol
IF protocol for DHRS1 antibody 16275-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols