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DHDH Polyclonal antibody

DHDH Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13900-1-AP

Synonyms

2DD, D xylose 1 dehydrogenase, D xylose NADP dehydrogenase, DHDH, HUM2DD

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using DHDH Polyclonal antibody (13900-1-AP)

    WB analysis of mouse testis using 13900-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13900-1-AP (DHDH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using DHDH Polyclonal antibody (13900-1-AP)

    WB analysis of mouse testis using 13900-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13900-1-AP (DHDH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DHDH Polyclonal antibody (13900-1-AP)

    IHC staining of human colon cancer using 13900-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 13900-1-AP (DHDH antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DHDH Polyclonal antibody (13900-1-AP)

    IHC staining of human colon cancer using 13900-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 13900-1-AP (DHDH antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13900-1-AP targets DHDH in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DHDH fusion protein Ag4866
Full Name dihydrodiol dehydrogenase (dimeric)
Calculated Molecular Weight 334 aa, 36 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC032730
Gene Symbol DHDH
Gene ID (NCBI) 27294
RRIDAB_2292833
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DHDH antibody 13900-1-APDownload protocol
IHC protocol for DHDH antibody 13900-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Proteomics

Quantitative proteomic analysis of oxaliplatin induced peripheral neurotoxicity.

Authors - Linlin Yang
View Article