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  • Featured Product
  • KD/KO Validated

DGKQ Polyclonal antibody

DGKQ Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 17885-1-AP

Synonyms

DGK-theta, DGKtheta, DGK theta, DAGK7, DAGK4

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using DGKQ Polyclonal antibody (17885-1-AP)

    WB analysis of mouse brain using 17885-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17885-1-AP (DGKQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DGKQ Polyclonal antibody (17885-1-AP)

    WB analysis using 17885-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 17885-1-AP (DGKQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using DGKQ Polyclonal antibody (17885-1-AP)

    WB analysis of human placenta using 17885-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 17885-1-AP (DGKQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DGKQ Polyclonal antibody (17885-1-AP)

    IHC staining of human colon cancer using 17885-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17885-1-AP (DGKQ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DGKQ Polyclonal antibody (17885-1-AP)

    IHC staining of human colon cancer using 17885-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17885-1-AP (DGKQ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DGKQ Polyclonal antibody (17885-1-AP)

    IHC staining of mouse brain using 17885-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17885-1-AP (DGKQ antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervix tissue using DGKQ Polyclonal antibody (17885-1-AP)

    IHC staining of human cervix using 17885-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervix using 17885-1-AP (DGKQ antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervix tissue using DGKQ Polyclonal antibody (17885-1-AP)

    IHC staining of human cervix using 17885-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervix using 17885-1-AP (DGKQ antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using DGKQ Polyclonal antibody (17885-1-AP)

    IHC staining of mouse brain using 17885-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17885-1-AP (DGKQ antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using DGKQ Polyclonal antibody (17885-1-AP)

    IF Staining of mouse small intestine using 17885-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using DGKQ antibody (17885-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using DGKQ Polyclonal antibody (17885-1-AP)

    IF Staining of mouse kidney using 17885-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using DGKQ antibody (17885-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse brain tissue, human placenta tissue, THP-1 cells
Positive IHC detected inhuman colon cancer tissue, human cervix tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse small intestine tissue, mouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17885-1-AP targets DGKQ in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DGKQ fusion protein Ag11938
Full Name diacylglycerol kinase, theta 110kDa
Calculated Molecular Weight 942 aa, 101 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC063801
Gene Symbol DGKQ
Gene ID (NCBI) 1609
RRIDAB_2091305
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DGKQ antibody 17885-1-APDownload protocol
IHC protocol for DGKQ antibody 17885-1-APDownload protocol
IF protocol for DGKQ antibody 17885-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Med Rep

Establishment of a novel hepatic steatosis cell model by Cas9/sgRNA-mediated DGKθ gene knockout.

Authors - Jingjing Zhang
  • KO Validated
View Article
WB

J Sci Food Agric

Neonatal resveratrol administration promotes skeletal muscle growth and insulin sensitivity in intrauterine growth-retarded suckling piglets associated with activation of FGF21-AMPKα pathway

Authors - Guangyi Bai
View Article