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  • Featured Product
  • KD/KO Validated

DGCR8 C-terminal Polyclonal antibody

DGCR8 C-terminal Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 10996-1-AP

Synonyms

DGCR8, C22orf12, pasha, LP4941, Gy1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DGCR8 C-terminal Polyclonal antibody (10996-1-AP)

    WB analysis using 10996-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10996-1-AP (DGCR8 C-terminal antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using DGCR8 C-terminal Polyclonal antibody (10996-1-AP)

    WB analysis of mouse testis using 10996-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 10996-1-AP (DGCR8 C-terminal antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using DGCR8 C-terminal Polyclonal antibody (10996-1-AP)

    WB analysis of A431 using 10996-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 10996-1-AP (DGCR8 C-terminal antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using DGCR8 C-terminal Polyclonal antibody (10996-1-AP)

    WB analysis of Jurkat using 10996-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10996-1-AP (DGCR8 C-terminal antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using DGCR8 C-terminal Polyclonal antibody (10996-1-AP)

    IP experiment of HEK-293 using 10996-1-AP

    IP result of anti-DGCR8 C-terminal (IP:10996-1-AP, 3ug; Detection:10996-1-AP 1:800) with HEK-293 cells lysate 2700ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DGCR8 C-terminal Polyclonal antibody (10996-1-AP)

    IHC staining of human breast cancer using 10996-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10996-1-AP (DGCR8 C-terminal antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DGCR8 C-terminal Polyclonal antibody (10996-1-AP)

    IHC staining of human breast cancer using 10996-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10996-1-AP (DGCR8 C-terminal antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DGCR8 C-terminal Polyclonal antibody (10996-1-AP)

    IHC staining of human colon cancer using 10996-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 10996-1-AP (DGCR8 C-terminal antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DGCR8 C-terminal Polyclonal antibody (10996-1-AP)

    IHC staining of human breast cancer using 10996-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10996-1-AP (DGCR8 C-terminal antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using DGCR8 C-terminal Polyclonal antibody (10996-1-AP)

    IF Staining of SH-SY5Y using 10996-1-AP

    Immunofluorescent analysis of SH-SY5Y cells, using DGCR8 antibody 10996-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Flow cytometry (FC) experiment of HeLa cells using DGCR8 C-terminal Polyclonal antibody (10996-1-AP)

    FC experiment of HeLa using 10996-1-AP

    1X10^6 HeLa cells were stained with 0.2ug DGCR8 C-terminal antibody (10996-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, mouse testis tissue, Jurkat cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10996-1-AP targets DGCR8 C-terminal in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DGCR8 C-terminal fusion protein Ag1429
Full Name DiGeorge syndrome critical region gene 8
Calculated Molecular Weight 773 aa, 86 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC009323
Gene Symbol DGCR8
Gene ID (NCBI) 54487
RRIDAB_2090987
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604]. The calculated molecular weight of DGCR8 is 82-86 kDa, but the post-modified DGCR8 is about 120 kDa (PMID: 18469815).

Protocols

Product Specific Protocols
WB protocol for DGCR8 C-terminal antibody 10996-1-APDownload protocol
IHC protocol for DGCR8 C-terminal antibody 10996-1-APDownload protocol
IF protocol for DGCR8 C-terminal antibody 10996-1-APDownload protocol
IP protocol for DGCR8 C-terminal antibody 10996-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB,IF

Nature

Global miRNA dosage control of embryonic germ layer specification.

Authors - Yingzi Cui
  • KO Validated
View Article
humanWB

Nature

Modulation of microRNA processing by p53.

Authors - Suzuki Hiroshi I HI
View Article
humanWB

Cell

Lin28A and Lin28B inhibit let-7 microRNA biogenesis by distinct mechanisms.

Authors - Piskounova Elena E
View Article
humanWB

Cell

Hippo signaling regulates microprocessor and links cell-density-dependent miRNA biogenesis to cancer.

Authors - Masaki Mori
View Article
mouseChIP

Cell

Super-Enhancer-Mediated RNA Processing Revealed by Integrative MicroRNA Network Analysis.

Authors - Hiroshi I Suzuki
View Article
humanWB

Cell Stem Cell

Principles of signaling pathway modulation for enhancing human naive pluripotency induction.

Authors - Jonathan Bayerl
View Article