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DCTPP1 Polyclonal antibody

DCTPP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16684-1-AP

Synonyms

CDA03, dCTP pyrophosphatase 1, dCTPase 1, DCTPP1, Deoxycytidine triphosphatase 1, RS21C6, XTP3TPA

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DCTPP1 Polyclonal antibody (16684-1-AP)

    WB analysis of HeLa using 16684-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16684-1-AP (DCTPP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using DCTPP1 Polyclonal antibody (16684-1-AP)

    WB analysis of mouse heart using 16684-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 16684-1-AP (DCTPP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Transfected HEK-293 cells using DCTPP1 Polyclonal antibody (16684-1-AP)

    WB analysis of Transfected HEK-293 using 16684-1-AP

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 16684-1-AP (DCTPP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using DCTPP1 Polyclonal antibody (16684-1-AP)

    IHC staining of human lung cancer using 16684-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16684-1-AP (DCTPP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using DCTPP1 Polyclonal antibody (16684-1-AP)

    IHC staining of human lung cancer using 16684-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16684-1-AP (DCTPP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DCTPP1 Polyclonal antibody (16684-1-AP)

    IF Staining of HeLa using 16684-1-AP

    Immunofluorescent analysis of HeLa cells using 16684-1-AP (DCTPP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, mouse heart tissue, Transfected HEK-293 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16684-1-AP targets DCTPP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DCTPP1 fusion protein Ag10015
Full Name dCTP pyrophosphatase 1
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 19 kDa, 40 kDa, 80 kDa
GenBank Accession NumberBC001344
Gene Symbol DCTPP1
Gene ID (NCBI) 79077
RRIDAB_2878299
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DCTPP1, also named as XTP3TPA, CDAO3 and RS21C6, is a hydrolyzes deoxynucleoside triphosphates (dNTPs) to the corresponding nucleoside monophosphates. It has a central role in the balance of dCTP and the metabolism of deoxycytidine analogues, thus contributing to the preservation of genome integrity. DCTPP1 has some forms such as monomer (~20 kDa), Dimer (~40 kDa) and Homotetramer (~80 kDa).

Protocols

Product Specific Protocols
WB protocol for DCTPP1 antibody 16684-1-APDownload protocol
IHC protocol for DCTPP1 antibody 16684-1-APDownload protocol
IF protocol for DCTPP1 antibody 16684-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

J Cell Mol Med

Expression of human dCTP pyrophosphatase 1 (DCTPP1) and its association with cisplatin resistance characteristics in ovarian cancer

Authors - Yu Wang
View Article