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DCTN1/p150 glued Polyclonal antibody

DCTN1/p150 glued Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, canine and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 55182-1-AP

Synonyms

DCTN1, p150 glued, DP-150, DCTN 1, DAP-150

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human brain tissue using p150 glued Polyclonal antibody (55182-1-AP)

    WB analysis of human brain using 55182-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 55182-1-AP (DCTN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using p150 glued Polyclonal antibody (55182-1-AP)

    WB analysis of Jurkat using 55182-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 55182-1-AP (DCTN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using p150 glued Polyclonal antibody (55182-1-AP)

    IHC staining of human brain using 55182-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 55182-1-AP (DCTN1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using p150 glued Polyclonal antibody (55182-1-AP)

    IHC staining of human brain using 55182-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 55182-1-AP (DCTN1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using DCTN1/p150 glued Polyclonal antibody (55182-1-AP)

    IF Staining of MDCK using 55182-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 55182-1-AP (p150 glued antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman brain tissue, Jurkat cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55182-1-AP targets DCTN1/p150 glued in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, canine samples.

Tested Reactivity human, canine
Cited Reactivityhuman, mussel
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name dynactin 1 (p150, glued homolog, Drosophila)
Calculated Molecular Weight 142 kDa
Observed Molecular Weight 135 kDa, 150 kDa
GenBank Accession NumberNM_004082
Gene Symbol p150 glued
Gene ID (NCBI) 1639
RRIDAB_10837074
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DCTN1, also named as DAP-150, p150-glued and p135, belongs to the dynactin 150 kDa subunit family. It is required for the cytoplasmic dynein-driven retrograde movement of vesicles and organelles along microtubules. Dynein-dynactin interaction is a key component of the mechanism of axonal transport of vesicles and organelles. Defects in DCTN1 are the cause of distal hereditary motor neuronopathy type 7B (HMN7B). This antibody is specific to DCTN1.

Protocols

Product Specific Protocols
WB protocol for DCTN1/p150 glued antibody 55182-1-APDownload protocol
IHC protocol for DCTN1/p150 glued antibody 55182-1-APDownload protocol
IF protocol for DCTN1/p150 glued antibody 55182-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
musselWB

Ecotoxicol Environ Saf

Proteomic profile in the mussel Perna viridis after short-term exposure to the brown tide alga Aureococcus anophagefferens.

Authors - Jian-Wei Zheng
View Article
humanIF

Science

The mechanism of acentrosomal spindle assembly in human oocytes

Authors - Tianyu Wu
View Article