DBT Polyclonal antibody
DBT Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 12451-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, mouse brain tissue, rat brain tissue, NCI-H1299 cells |
| Positive IHC detected in | human stomach cancer tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1500-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IHC | See 1 publications below |
| IP | See 1 publications below |
Product Information
12451-1-AP targets DBT in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DBT fusion protein Ag3101 |
| Full Name | dihydrolipoamide branched chain transacylase E2 |
| Calculated Molecular Weight | 482 aa, 53 kDa |
| Observed Molecular Weight | 53 kDa |
| GenBank Accession Number | BC016675 |
| Gene Symbol | DBT |
| Gene ID (NCBI) | 1629 |
| RRID | AB_2089636 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DBT antibody 12451-1-AP | Download protocol |
| IHC protocol for DBT antibody 12451-1-AP | Download protocol |
| IF protocol for DBT antibody 12451-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Metab Branched-chain amino acid catabolism in muscle affects systemic BCAA levels but not insulin resistance | ||
J Nutr Pyruvate Kinase M2 Supports Muscle Progenitor Cell Proliferation but Is Dispensable for Skeletal Muscle Regeneration after Injury. | ||
Heliyon Hyperactivation of mTOR and AKT in a cardiac hypertrophy animal model of Friedreich ataxia | ||
Cell Metab Extra-cardiac BCAA catabolism lowers blood pressure and protects from heart failure | ||
Front Immunol Overexpression of DBT suppresses the aggressiveness of renal clear cell carcinoma and correlates with immune infiltration | ||
J Thromb Haemost Branched-chain amino acids promote thrombocytopoiesis by activating mTOR signaling |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Maria (Verified Customer) (08-15-2021) | DBT in human primary fibroblasts. 10 ug of total protein. 4-15% TGX gel. Blocking 3% BSA in PBST (0.1% Tween). 1:1000 primary ab O/N incubation at 4ºC. 1:5000 secondary ab HRP conj. 1h RT in BSA 3% in PBST.
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