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  • Featured Product
  • KD/KO Validated

HIP55 Polyclonal antibody

HIP55 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 13015-1-AP

Synonyms

DBNL, HPK1-interacting protein of 55 kDa, Drebrin-like protein, Drebrin-F, Cervical mucin-associated protein

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using HIP55 Polyclonal antibody (13015-1-AP)

    WB analysis of mouse brain using 13015-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13015-1-AP (DBNL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using HIP55 Polyclonal antibody (13015-1-AP)

    WB analysis of mouse heart using 13015-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 13015-1-AP (DBNL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HIP55 Polyclonal antibody (13015-1-AP)

    WB analysis of HeLa using 13015-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13015-1-AP (DBNL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using HIP55 Polyclonal antibody (13015-1-AP)

    WB analysis of human heart using 13015-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 13015-1-AP (DBNL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using HIP55 Polyclonal antibody (13015-1-AP)

    WB analysis of K-562 using 13015-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13015-1-AP (DBNL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using HIP55 Polyclonal antibody (13015-1-AP)

    WB analysis of Jurkat using 13015-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13015-1-AP (DBNL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using HIP55 Polyclonal antibody (13015-1-AP)

    IP experiment of mouse brain using 13015-1-AP

    IP result of anti-HIP55 (IP:13015-1-AP, 4ug; Detection:13015-1-AP 1:1000) with mouse brain tissue lysate 2640ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HIP55 Polyclonal antibody (13015-1-AP)

    IHC staining of human breast cancer using 13015-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13015-1-AP (DBNL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HIP55 Polyclonal antibody (13015-1-AP)

    IHC staining of human breast cancer using 13015-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13015-1-AP (DBNL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HIP55 Polyclonal antibody (13015-1-AP)

    IF Staining of HepG2 using 13015-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HIP55 antibody (13015-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HIP55 Polyclonal antibody (13015-1-AP)

    IF Staining of HepG2 using 13015-1-AP

    Immunofluorescent analysis of HepG2 cells, using DBNL antibody 13015-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green).

  • Flow cytometry (FC) experiment of HepG2 cells using HIP55 Polyclonal antibody (13015-1-AP)

    FC experiment of HepG2 using 13015-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug HIP55 Polyclonal antibody (13015-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, human heart tissue, Jurkat cells, K-562 cells, mouse heart tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13015-1-AP targets HIP55 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HIP55 fusion protein Ag4078
Full Name drebrin-like
Calculated Molecular Weight 430 aa, 48 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC031687
Gene Symbol HIP55
Gene ID (NCBI) 28988
RRIDAB_2230306
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HIP55, also known as DBNL, ABP1, SH3P7, belongs to the ABP1 family. HIP55 plays a role in the reorganization of the actin cytoskeleton, formation of cell projections, such as neurites, in neuron morphogenesis and synapse formation via its interaction with WASL and COBL. HIP55 acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes (PMID: 14729663). The N terminus of HIP55 contains a putative actin-binding domain found in drebrins, which are involved in brain development, and the C terminus contains an SH3 domain. Expect a band 55 kDa in size corresponding to HIP55 by western blotting.

Protocols

Product Specific Protocols
WB protocol for HIP55 antibody 13015-1-APDownload protocol
IHC protocol for HIP55 antibody 13015-1-APDownload protocol
IF protocol for HIP55 antibody 13015-1-APDownload protocol
IP protocol for HIP55 antibody 13015-1-APDownload protocol
FC protocol for HIP55 antibody 13015-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IP

Acta Pharmacol Sin

The multifunctional adaptor protein HIP-55 couples Smad7 to accelerate TGF-β type I receptor degradation.

Authors - Yang Sun
  • KD Validated
View Article
mouseWB,IF,IP

J Neurosci

Drebrin-like (Dbnl) controls neuronal migration via regulating N-cadherin expression in the developing cerebral cortex.

Authors - Seika Inoue
View Article

J Cell Sci

VEGF-A stimulates podosome-mediated collagen-IV proteolysis in microvascular endothelial cells.

Authors - Thomas Daubon
View Article
mouseWB

J Microbiol

Effect of Salmonella treatment on an implanted tumor (CT26) in a mouse model.

Authors - Yun Misun M
View Article
humanIF

Nature

A cross-species proteomic map reveals neoteny of human synapse development

Authors - Li Wang
View Article