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BRINP1 Monoclonal antibody

BRINP1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2c

Reactivity

human, pig, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A10E3

Cat no : 60336-1-Ig

Synonyms

BRINP1, DBC1, DBCCR1, deleted in bladder cancer 1, FAM5A, Protein FAM5A

Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using BRINP1 Monoclonal antibody (60336-1-Ig)

    WB analysis of fetal human brain using 60336-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60336-1-Ig (DBC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using BRINP1 Monoclonal antibody (60336-1-Ig)

    WB analysis of mouse brain using 60336-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 60336-1-Ig (DBC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using BRINP1 Monoclonal antibody (60336-1-Ig)

    WB analysis of pig brain using 60336-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 60336-1-Ig (DBC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using BRINP1 Monoclonal antibody (60336-1-Ig)

    IF Staining of MCF-7 using 60336-1-Ig

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed MCF-7 cells using 60336-1-Ig(DBC1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected infetal human brain tissue, mouse brain tissue, pig brain tissue
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60336-1-Ig targets BRINP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, pig, mouse samples.

Tested Reactivity human, pig, mouse
Host / Isotype Mouse / IgG2c
Class Monoclonal
Type Antibody
Immunogen BRINP1 fusion protein Ag6588
Full Name deleted in bladder cancer 1
Calculated Molecular Weight 761 aa, 88 kDa
Observed Molecular Weight 88 kDa
GenBank Accession NumberBC021560
Gene Symbol BRINP1
Gene ID (NCBI) 1620
RRIDAB_2881445
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BRINP1, also known as DBC1 and FAM5A, inhibits cell proliferation by negative regulation of the G1/S transition. It mediates cell death which is not of the classical apoptotic type and regulates expression of components of the plasminogen pathway.

Protocols

Product Specific Protocols
WB protocol for BRINP1 antibody 60336-1-IgDownload protocol
IF protocol for BRINP1 antibody 60336-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols