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DAX-1 Polyclonal antibody

DAX-1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20849-1-AP

Synonyms

AHC, AHCH, AHX, DAX 1, DAX1, DSS, GTD, HHG, NR0B1, NROB1, Nuclear receptor DAX 1

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using DAX-1 Polyclonal antibody (20849-1-AP)

    WB analysis of MCF-7 using 20849-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 20849-1-AP (DAX-1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using DAX-1 Polyclonal antibody (20849-1-AP)

    IHC staining of human testis using 20849-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20849-1-AP (DAX-1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using DAX-1 Polyclonal antibody (20849-1-AP)

    IHC staining of human small intestine using 20849-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20849-1-AP (DAX-1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using DAX-1 Polyclonal antibody (20849-1-AP)

    IHC staining of human testis using 20849-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20849-1-AP (DAX-1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using DAX-1 Polyclonal antibody (20849-1-AP)

    IHC staining of human small intestine using 20849-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20849-1-AP (DAX-1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using DAX-1 Polyclonal antibody (20849-1-AP)

    IF Staining of MCF-7 using 20849-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 20849-1-AP (DAX-1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman small intestine tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20849-1-AP is DAX-1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DAX-1 fusion protein Ag14416
Full Name nuclear receptor subfamily 0, group B, member 1
Calculated Molecular Weight 470 aa, 52 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC011564
Gene Symbol NR0B1
Gene ID (NCBI) 190
RRIDAB_2878751
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DAX-1 antibody 20849-1-APDownload protocol
IHC protocol for DAX-1 antibody 20849-1-APDownload protocol
IF protocol for DAX-1 antibody 20849-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols