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ZIP kinase Polyclonal antibody

ZIP kinase Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19540-1-AP

Synonyms

DAPK3, Dlk, Death-associated protein kinase 3, DAP-like kinase, DAP like kinase

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ZIP kinase Polyclonal antibody (19540-1-AP)

    IHC staining of human stomach cancer using 19540-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19540-1-AP (ZIP kinase antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using ZIP kinase Polyclonal antibody (19540-1-AP)

    IHC staining of rat colon using 19540-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 19540-1-AP (ZIP kinase antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat heart tissue using ZIP kinase Polyclonal antibody (19540-1-AP)

    IHC staining of rat heart using 19540-1-AP

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 19540-1-AP (ZIP kinase antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using ZIP kinase Polyclonal antibody (19540-1-AP)

    IHC staining of mouse colon using 19540-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 19540-1-AP (ZIP kinase antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ZIP kinase Polyclonal antibody (19540-1-AP)

    IF Staining of HeLa using 19540-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 19540-1-AP (ZIP kinase antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using ZIP kinase Polyclonal antibody (19540-1-AP)

    IF Staining of U-251 using 19540-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using ZIP kinase antibody (19540-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive IHC detected inhuman stomach cancer tissue, rat colon tissue, rat heart tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, U-251 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19540-1-AP targets ZIP kinase in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name death-associated protein kinase 3
Calculated Molecular Weight 53 kDa
Observed Molecular Weight37, 52 kDa
GenBank Accession NumberNM_001348
Gene Symbol DAPK3
Gene ID (NCBI) 1613
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zipper-interacting protein (ZIP) kinase, also known as death-associated protein kinase 3 (DAPK3), is a 

serine/threonine kinase that positively regulates mitochondrial-dependent apoptosis as well as autophagy 

and acts as a tumor suppressor.


What is the molecular weight of ZIP kinase? Is ZIP kinase post-translationally modified?

The molecular weight of ZIP kinase is 52 kDa. ZIP kinase contains seven phosphorylation sites (six threonine 

and one serine residues) that control its activity and localization (PMID: 24193917).


What is the subcellular localization of ZIP kinase?

ZIP kinase contains nuclear localization signal in its C-terminal part. ZIP kinase is present both in the cytoplasm 

and in the nucleus. In the cytoplasm ZIP kinase interacts with actin cytoskeleton, regulating contraction of 

smooth muscles (PMID: 16005610) and membrane blebbing (PMID: 11781833). In the nucleus it interacts 

with several transcription factors regulating gene expression.


What is the tissue expression pattern of ZIP kinase?

ZIP kinase is ubiquitously expressed.


Protocols

Product Specific Protocols
IHC protocol for ZIP kinase antibody 19540-1-APDownload protocol
IF protocol for ZIP kinase antibody 19540-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols