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DALRD3 Polyclonal antibody

DALRD3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26294-1-AP

Synonyms

DALRD3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of COLO 320 cells using DALRD3 Polyclonal antibody (26294-1-AP)

    WB analysis of COLO 320 using 26294-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 26294-1-AP (DALRD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using DALRD3 Polyclonal antibody (26294-1-AP)

    WB analysis of K-562 using 26294-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 26294-1-AP (DALRD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DALRD3 Polyclonal antibody (26294-1-AP)

    IHC staining of human colon cancer using 26294-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26294-1-AP (DALRD3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using DALRD3 Polyclonal antibody (26294-1-AP)

    IHC staining of human colon cancer using 26294-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26294-1-AP (DALRD3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inK-562 cells, COLO 320 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

26294-1-AP targets DALRD3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DALRD3 fusion protein Ag23437
Full Name DALR anticodon binding domain containing 3
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC047683
Gene Symbol DALRD3
Gene ID (NCBI) 55152
RRIDAB_2880467
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DALRD3 antibody 26294-1-APDownload protocol
IHC protocol for DALRD3 antibody 26294-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

DALRD3 encodes a protein mutated in epileptic encephalopathy that targets arginine tRNAs for 3-methylcytosine modification.

Authors - Jenna M Lentini
View Article