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  • Featured Product
  • KD/KO Validated

DAK Polyclonal antibody

DAK Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 12224-1-AP

Synonyms

FAD-AMP lyase, DHA kinase, cyclizing, cyclic FMN forming, Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using DAK Polyclonal antibody (12224-1-AP)

    WB analysis of HeLa using 12224-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12224-1-AP (DAK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DAK Polyclonal antibody (12224-1-AP)

    WB analysis of HeLa using 12224-1-AP

    WB result of DAK antibody (12224-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-DAK transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using DAK Polyclonal antibody (12224-1-AP)

    WB analysis using 12224-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12224-1-AP (DAK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using DAK Polyclonal antibody (12224-1-AP)

    WB analysis of HEK-293 using 12224-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12224-1-AP (DAK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse uterus tissue using DAK Polyclonal antibody (12224-1-AP)

    WB analysis of mouse uterus using 12224-1-AP

    mouse uterus tissue were subjected to SDS PAGE followed by western blot with 12224-1-AP (DAK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DAK Polyclonal antibody (12224-1-AP)

    WB analysis of HeLa using 12224-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12224-1-AP (DAK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using DAK Polyclonal antibody (12224-1-AP)

    IP experiment of HeLa using 12224-1-AP

    IP result of anti-DAK (IP:12224-1-AP, 3ug; Detection:12224-1-AP 1:500) with HeLa cells lysate 2150ug.

  • Immunohistochemistry (IHC) staining of human stomach tissue using DAK Polyclonal antibody (12224-1-AP)

    IHC staining of human stomach using 12224-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12224-1-AP (DAK antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, mouse uterus tissue, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12224-1-AP targets DAK in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DAK fusion protein Ag2861
Full Name dihydroxyacetone kinase 2 homolog (S. cerevisiae)
Calculated Molecular Weight 575 aa, 59 kDa
Observed Molecular Weight 55-59 kDa
GenBank Accession NumberBC001341
Gene Symbol DAK
Gene ID (NCBI) 26007
RRIDAB_2277108
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DAK, also name as TKFC, Triokinase, and FMN cyclase, catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate. DAK has some isoforms with MW 51-55 kDa and 59 kDa.

Protocols

Product Specific Protocols
WB protocol for DAK antibody 12224-1-APDownload protocol
IHC protocol for DAK antibody 12224-1-APDownload protocol
IP protocol for DAK antibody 12224-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Endocrinology

Hepatic but not intestinal FBP1 is required for fructose metabolism and tolerance

Authors - Qiufang Bai
View Article
humanWB,IF

iScience

CircSOBP suppresses the progression of glioma by disrupting glycolysis and promoting the MDA5-mediated immune response

Authors - Maolin Mu
  • KO Validated
View Article
ratWB

Mol Psychiatry

Proteasome-independent K63 polyubiquitination selectively regulates ATP levels and proteasome activity during fear memory formation in the female amygdala

Authors - Kayla Farrell
View Article