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CYP2C19 Polyclonal antibody

CYP2C19 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 55453-1-AP

Synonyms

(R) limonene 6 monooxygenase, (S) limonene 6 monooxygenase, (S) limonene 7 monooxygenase, CPCJ, CYP2C, CYP2C19, CYPIIC17, CYPIIC19, Cytochrome P450 11A, Cytochrome P450 254C, Cytochrome P450 2C19, Mephenytoin 4 hydroxylase, P450C2C, P450IIC19

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP2C19 Polyclonal antibody (55453-1-AP)

    IHC staining of human liver cancer using 55453-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55453-1-AP (CYP2C19 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP2C19 Polyclonal antibody (55453-1-AP)

    IHC staining of human liver cancer using 55453-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55453-1-AP (CYP2C19 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CYP2C19 Polyclonal antibody (55453-1-AP)

    IF Staining of HeLa using 55453-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 55453-1-AP (CYP2C19 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55453-1-AP targets CYP2C19 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name cytochrome P450, family 2, subfamily C, polypeptide 19
Calculated Molecular Weight 56 kDa
GenBank Accession NumberNM_000769
Gene Symbol CYP2C19
Gene ID (NCBI) 1557
RRIDAB_2881340
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for CYP2C19 antibody 55453-1-APDownload protocol
IF protocol for CYP2C19 antibody 55453-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols