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CYP11B2 Polyclonal antibody

CYP11B2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20968-1-AP

Synonyms

CYP11BL, CYP11B, CPN2, Corticosterone 18-monooxygenase, CYP11B2, Aldosterone-synthesizing enzyme

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CYP11B2 Polyclonal antibody (20968-1-AP)

    WB analysis using 20968-1-AP

    pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 20968-1-AP (CYP11B2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SKOV-3 cells using CYP11B2 Polyclonal antibody (20968-1-AP)

    WB analysis of SKOV-3 using 20968-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 20968-1-AP (CYP11B2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human adrenal gland tissue using CYP11B2 Polyclonal antibody (20968-1-AP)

    IHC staining of human adrenal gland using 20968-1-AP

    Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue slide using 20968-1-AP (CYP11B2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human adrenal gland tissue using CYP11B2 Polyclonal antibody (20968-1-AP)

    IHC staining of human adrenal gland using 20968-1-AP

    Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue slide using 20968-1-AP (CYP11B2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP11B2 Polyclonal antibody (20968-1-AP)

    IHC staining of human liver cancer using 20968-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CYP11B2 Polyclonal antibody (20968-1-AP)

    IHC staining of human liver cancer using 20968-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CYP11B2 Polyclonal antibody (20968-1-AP)

    IHC staining of human kidney using 20968-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CYP11B2 Polyclonal antibody (20968-1-AP)

    IHC staining of human kidney using 20968-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using CYP11B2 Polyclonal antibody (20968-1-AP)

    IHC staining of human hepatocirrhosis using 20968-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using CYP11B2 Polyclonal antibody (20968-1-AP)

    IHC staining of human hepatocirrhosis using 20968-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of SKOV-3 cells using CYP11B2 Polyclonal antibody (20968-1-AP)

    IF Staining of SKOV-3 using 20968-1-AP

    Immunofluorescent analysis of SKOV-3 cells using 20968-1-AP (CYP11B2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inSKOV-3 cells, pig adrenal gland tissue
Positive IHC detected inhuman adrenal gland tissue, human kidney tissue, human hepatocirrhosis tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSKOV-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20968-1-AP targets CYP11B2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name cytochrome P450, family 11, subfamily B, polypeptide 2
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 48-50 kDa
GenBank Accession NumberNM_000498
Gene Symbol CYP11B2
Gene ID (NCBI) 1585
RRIDAB_2878777
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYP11B2 (Cytochrome P450 11B2), also known as aldosterone synthase, is a member of the cytochrome P450 superfamily of enzymes. CYP11B2 can only catalyze 11-deoxycorticosterone oxidation to produce corticosterone and further hydroxylation to ALD. CYP11B2 is an enzyme required for cortisol biosynthesis and is specifically expressed in aldosterone-producing tissue of the adrenal cortex and is overexpressed in aldosterone-producing adenomas (APA).

Protocols

Product Specific Protocols
WB protocol for CYP11B2 antibody 20968-1-APDownload protocol
IHC protocol for CYP11B2 antibody 20968-1-APDownload protocol
IF protocol for CYP11B2 antibody 20968-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Nat Commun

Adrenal tropism of SARS-CoV-2 and adrenal findings in a post-mortem case series of patients with severe fatal COVID-19.

Authors - Tanja Paul
View Article
humanWB,IHC

Cell Death Dis

Aldosterone from endometrial glands is benefit for human decidualization.

Authors - Shu-Yun Li
View Article
mouseIF

PeerJ

Adrenal SGLT1 or SGLT2 as predictors of atherosclerosis under chronic stress based on a computer algorithm

Authors - Jianyi Li
View Article