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CTRB1 Polyclonal antibody

CTRB1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24939-1-AP

Synonyms

chymotrypsinogen B1, CTRB, CTRB1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse pancreas tissue using CTRB1 Polyclonal antibody (24939-1-AP)

    WB analysis of mouse pancreas using 24939-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 24939-1-AP (CTRB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat pancreas tissue using CTRB1 Polyclonal antibody (24939-1-AP)

    WB analysis of rat pancreas using 24939-1-AP

    rat pancreas tissue were subjected to SDS PAGE followed by western blot with 24939-1-AP (CTRB1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat pancreas tissue using CTRB1 Polyclonal antibody (24939-1-AP)

    WB analysis of rat pancreas using 24939-1-AP

    rat pancreas tissue were subjected to SDS PAGE followed by western blot with 24939-1-AP (CTRB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using CTRB1 Polyclonal antibody (24939-1-AP)

    WB analysis of mouse pancreas using 24939-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 24939-1-AP (CTRB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CTRB1 Polyclonal antibody (24939-1-AP)

    IHC staining of human pancreas using 24939-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24939-1-AP (CTRB1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CTRB1 Polyclonal antibody (24939-1-AP)

    IHC staining of human pancreas using 24939-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24939-1-AP (CTRB1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CTRB1 Polyclonal antibody (24939-1-AP)

    IHC staining of human pancreas using 24939-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24939-1-AP (CTRB1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CTRB1 Polyclonal antibody (24939-1-AP)

    IHC staining of human pancreas using 24939-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24939-1-AP (CTRB1 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inmouse pancreas tissue, rat pancreas tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24939-1-AP is CTRB1 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CTRB1 fusion protein Ag8604
Full Name chymotrypsinogen B1
Calculated Molecular Weight 263 aa, 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC005385
Gene Symbol CTRB1
Gene ID (NCBI) 1504
RRIDAB_2879809
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CTRB1 antibody 24939-1-APDownload protocol
IHC protocol for CTRB1 antibody 24939-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB，IF

Biol Open

Exocrine pancreas proteases regulate β-cell proliferation in zebrafish ciliopathy models and in murine systems.

Authors - Timothy L Hostelley
View Article