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CTR9 Polyclonal antibody

CTR9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21264-1-AP

Synonyms

CTR9, KIAA0155, p150, p150TSP, SH2 domain binding protein 1, SH2BP1, TSBP

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using CTR9 Polyclonal antibody (21264-1-AP)

    WB analysis of Raji using 21264-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 21264-1-AP (CTR9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using CTR9 Polyclonal antibody (21264-1-AP)

    WB analysis of mouse lung using 21264-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 21264-1-AP (CTR9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CTR9 Polyclonal antibody (21264-1-AP)

    WB analysis of HeLa using 21264-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21264-1-AP (CTR9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CTR9 Polyclonal antibody (21264-1-AP)

    WB analysis of HEK-293 using 21264-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21264-1-AP (CTR9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using CTR9 Polyclonal antibody (21264-1-AP)

    WB analysis of L02 using 21264-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 21264-1-AP (CTR9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using CTR9 Polyclonal antibody (21264-1-AP)

    WB analysis of mouse testis using 21264-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 21264-1-AP (CTR9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CTR9 Polyclonal antibody (21264-1-AP)

    IHC staining of human breast cancer using 21264-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 21264-1-AP (CTR9 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CTR9 Polyclonal antibody (21264-1-AP)

    IHC staining of human breast cancer using 21264-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 21264-1-AP (CTR9 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CTR9 Polyclonal antibody (21264-1-AP)

    IHC staining of human colon cancer using 21264-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 21264-1-AP (CTR9 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CTR9 Polyclonal antibody (21264-1-AP)

    IHC staining of human colon cancer using 21264-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 21264-1-AP (CTR9 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CTR9 Polyclonal antibody (21264-1-AP)

    IF Staining of HeLa using 21264-1-AP

    Immunofluorescent analysis of Hela cells, using CTR9 antibody 21264-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inRaji cells, HEK-293 cells, HeLa cells, L02 cells, mouse lung tissue, mouse testis tissue
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21264-1-AP is CTR9 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CTR9 fusion protein Ag15690
Full Name Ctr9, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
Calculated Molecular Weight 1173 aa, 134 kDa
Observed Molecular Weight 130-150 kDa
GenBank Accession NumberBC058914
Gene Symbol CTR9
Gene ID (NCBI) 9646
RRIDAB_10734585
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CTR9 antibody 21264-1-APDownload protocol
IHC protocol for CTR9 antibody 21264-1-APDownload protocol
IF protocol for CTR9 antibody 21264-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Autophagy

The Paf1 complex transcriptionally regulates the mitochondrial-anchored protein Atg32 leading to activation of mitophagy.

Authors - Liangde Zheng
View Article
mouseWB

Front Pharmacol

Tetrandrine Prevents Bone Loss in Ovariectomized Mice by Inhibiting RANKL-Induced Osteoclastogenesis.

Authors - Zeyuan Zhong
View Article
mouseWB

Cell Death Dis

UBR5 targets tumor suppressor CDC73 proteolytically to promote aggressive breast cancer.

Authors - Gang Xiang
View Article
humanWB

Cell

SARS-CoV-2 variants evolve convergent strategies to remodel the host response

Authors - Mehdi Bouhaddou
View Article
humanIHC

Eur J Histochem

MicroRNA-17-3p is upregulated in psoriasis and regulates keratinocyte hyperproliferation and pro-inflammatory cytokine secretion by targeting CTR9

Authors - Qingwen Li
View Article