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Alpha N-Catenin Polyclonal antibody

Alpha N-Catenin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14362-1-AP

Synonyms

CTNNA2, Catenin alpha-2, Catenin alpha 2, CAPR, Alpha-catenin-related protein

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using Alpha N-Catenin Polyclonal antibody (14362-1-AP)

    WB analysis of SH-SY5Y using 14362-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14362-1-AP (alpha N Catenin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using Alpha N-Catenin Polyclonal antibody (14362-1-AP)

    WB analysis of mouse pancreas using 14362-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14362-1-AP (alpha N Catenin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of SH-SY5Y cells using Alpha N-Catenin Polyclonal antibody (14362-1-AP)

    IP experiment of SH-SY5Y using 14362-1-AP

    IP result of anti-Alpha N-Catenin (IP:14362-1-AP, 4ug; Detection:14362-1-AP 1:500) with SH-SY5Y cells lysate 1600ug.

  • Immunohistochemistry (IHC) staining of rat brain tissue using Alpha N-Catenin Polyclonal antibody (14362-1-AP)

    IHC staining of rat brain using 14362-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14362-1-AP (Alpha N-Catenin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using Alpha N-Catenin Polyclonal antibody (14362-1-AP)

    IHC staining of rat brain using 14362-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14362-1-AP (Alpha N-Catenin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using Alpha N-Catenin Polyclonal antibody (14362-1-AP)

    IF Staining of SH-SY5Y using 14362-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Alpha N-Catenin antibody (14362-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inSH-SY5Y cells, mouse pancreas tissue
Positive IP detected inSH-SY5Y cells
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14362-1-AP targets Alpha N-Catenin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha N-Catenin fusion protein Ag5747
Full Name catenin (cadherin-associated protein), alpha 2
Calculated Molecular Weight 105 kDa
Observed Molecular Weight 102 kDa
GenBank Accession NumberBC052996
Gene Symbol Alpha N-Catenin
Gene ID (NCBI) 1496
RRIDAB_2087961
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Alpha N-Catenin antibody 14362-1-APDownload protocol
IHC protocol for Alpha N-Catenin antibody 14362-1-APDownload protocol
IF protocol for Alpha N-Catenin antibody 14362-1-APDownload protocol
IP protocol for Alpha N-Catenin antibody 14362-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Endocrinology

The planar cell polarity (PCP) protein Fat 1 in Sertoli cell function

Authors - Tiao Bu
View Article
human

Neuron

Nuclear aggregates of NONO/SFPQ and A-to-I-edited RNA in Parkinson's disease and dementia with Lewy bodies

Authors - Nandkishore R Belur
View Article