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FLJ23584 Polyclonal antibody

FLJ23584 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21143-1-AP

Synonyms

C22orf46, CTA 216E10.6, CTA-216E10.6, FLJ23584, hypothetical FLJ23584

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using FLJ23584 Polyclonal antibody (21143-1-AP)

    WB analysis of HepG2 using 21143-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21143-1-AP (FLJ23584 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using FLJ23584 Polyclonal antibody (21143-1-AP)

    WB analysis of human brain using 21143-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 21143-1-AP (C22orf46 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using FLJ23584 Polyclonal antibody (21143-1-AP)

    WB analysis of mouse liver using 21143-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 21143-1-AP (C22orf46 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using FLJ23584 Polyclonal antibody (21143-1-AP)

    WB analysis of L02 using 21143-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 21143-1-AP (C22orf46 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using FLJ23584 Polyclonal antibody (21143-1-AP)

    IHC staining of human brain using 21143-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21143-1-AP (FLJ23584 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using FLJ23584 Polyclonal antibody (21143-1-AP)

    IHC staining of human brain using 21143-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21143-1-AP (FLJ23584 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using FLJ23584 Polyclonal antibody (21143-1-AP)

    IHC staining of human testis using 21143-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21143-1-AP (C22orf46 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using FLJ23584 Polyclonal antibody (21143-1-AP)

    IHC staining of human testis using 21143-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21143-1-AP (C22orf46 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FLJ23584 Polyclonal antibody (21143-1-AP)

    IF Staining of HepG2 using 21143-1-AP

    Immunofluorescent analysis of HepG2 cells using 21143-1-AP (FLJ23584 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, human brain tissue, L02 cells, mouse liver tissue
Positive IHC detected inhuman brain tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21143-1-AP is FLJ23584 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FLJ23584 fusion protein Ag15647
Full Name hypothetical FLJ23584
Calculated Molecular Weight 234 aa, 26 kDa
Observed Molecular Weight 28-30 kDa
GenBank Accession NumberBC007210
Gene Symbol FLJ23584
Gene ID (NCBI) 79640
RRIDAB_10732689
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FLJ23584 is coded by the mRNA of chromosome 22 open reading frame 46. The reading frame is different to C22orf46. This antibody detects the hypothetical FLJ23584 protein.

Protocols

Product Specific Protocols
WB protocol for FLJ23584 antibody 21143-1-APDownload protocol
IHC protocol for FLJ23584 antibody 21143-1-APDownload protocol
IF protocol for FLJ23584 antibody 21143-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols