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CRYBB1 Monoclonal antibody

CRYBB1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

6F12B12

Cat no : 60273-1-Ig

Synonyms

Beta B1 crystallin, Beta crystallin B1, CATCN3, CRYBB1, crystallin, beta B1

Validation Data Gallery

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  • Western Blot (WB) analysis of C6 cells using CRYBB1 Monoclonal antibody (60273-1-Ig)

    WB analysis of C6 using 60273-1-Ig

    C6 cells were subjected to SDS PAGE followed by western blot with 60273-1-Ig (CRYBB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse eye tissue using CRYBB1 Monoclonal antibody (60273-1-Ig)

    WB analysis of mouse eye using 60273-1-Ig

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 60273-1-Ig (CRYBB1 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using CRYBB1 Monoclonal antibody (60273-1-Ig)

    IF Staining of hTERT-RPE1 using 60273-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CRYBB1 antibody (60273-1-Ig, Clone: 6F12B12 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inC6 cells, mouse eye tissue
Positive IF/ICC detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60273-1-Ig targets CRYBB1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CRYBB1 fusion protein Ag4722
Full Name crystallin, beta B1
Calculated Molecular Weight 252 aa, 28 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC036790
Gene Symbol CRYBB1
Gene ID (NCBI) 1414
RRIDAB_2881393
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mutation in CRYBB1 is associated with congenital Cataract-Microcornea Syndrome.

Protocols

Product Specific Protocols
WB protocol for CRYBB1 antibody 60273-1-IgDownload protocol
IF protocol for CRYBB1 antibody 60273-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols