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CRYBA2 Polyclonal antibody

CRYBA2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 15750-1-AP

Synonyms

Beta A2 crystallin, Beta crystallin A2, CRYBA2, crystallin, beta A2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CRYBA2 Polyclonal antibody (15750-1-AP)

    WB analysis using 15750-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15750-1-AP (CRYBA2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat eye tissue using CRYBA2 Polyclonal antibody (15750-1-AP)

    WB analysis of rat eye using 15750-1-AP

    rat eye tissue were subjected to SDS PAGE followed by western blot with 15750-1-AP (CRYBA2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse eye tissue using CRYBA2 Polyclonal antibody (15750-1-AP)

    WB analysis of mouse eye using 15750-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 15750-1-AP (CRYBA2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse eye tissue, rat eye tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15750-1-AP targets CRYBA2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRYBA2 fusion protein Ag8222
Full Name crystallin, beta A2
Calculated Molecular Weight 197 aa, 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC006285
Gene Symbol CRYBA2
Gene ID (NCBI) 1412
RRIDAB_2878180
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRYBA2, also named as Beta A2 crystallin, is a member of crystallin superfamily. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also defined as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Crystallins are the dominant structural components of the vertebrate eye lens. The MW of this protein is 22 kDa, and this antibody specially recognises the 22 kDa protein.

Protocols

Product Specific Protocols
WB protocol for CRYBA2 antibody 15750-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols