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  • Featured Product
  • KD/KO Validated

Cryptochrome 1 Polyclonal antibody

Cryptochrome 1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13474-1-AP

Synonyms

CRY1, Cryptochrome-1, CRY 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Cryptochrome 1 Polyclonal antibody (13474-1-AP)

    WB analysis using 13474-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13474-1-AP (Cryptochrome 1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Cryptochrome 1 Polyclonal antibody (13474-1-AP)

    IHC staining of mouse brain using 13474-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13474-1-AP (Cryptochrome 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cryptochrome 1 Polyclonal antibody (13474-1-AP)

    IHC staining of human breast cancer using 13474-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13474-1-AP (Cryptochrome 1 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Cryptochrome 1 Polyclonal antibody (13474-1-AP)

    IHC staining of human breast cancer using 13474-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13474-1-AP (Cryptochrome 1 antibody at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Cryptochrome 1 Polyclonal antibody (13474-1-AP)

    IF Staining of HeLa using 13474-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cryptochrome 1 antibody (13474-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Cryptochrome 1 Polyclonal antibody (13474-1-AP)

    IF Staining of MCF-7 using 13474-1-AP

    Immunofluorescent analysis of MCF-7 cells, using CRY1 antibody 13474-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Flow cytometry (FC) experiment of HeLa cells using Cryptochrome 1 Polyclonal antibody (13474-1-AP)

    FC experiment of HeLa using 13474-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cryptochrome 1 (13474-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHeLa cells, Y79 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse brain tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, MCF-7 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13474-1-AP targets Cryptochrome 1 in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cryptochrome 1 fusion protein Ag4270
Full Name cryptochrome 1 (photolyase-like)
Calculated Molecular Weight 586 aa, 66 kDa
Observed Molecular Weight60-66 kDa
GenBank Accession NumberBC030519
Gene Symbol CRY1
Gene ID (NCBI) 1407
RRIDAB_10697652
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cryptochrome circadian clock 1 (CRY1) is a flavin adenine dinucleotide-binding protein with MW of 66 kDa. CRY1 is a key component of the circadian core oscillator complex, which regulates the circadian clock. CRY1 predominantly displays evening-time expression and serves as a strong repressor of morning-time elements (E box/E-prime box) when bound to the BMAL1 (ARNTL; 602550)/CLOCK (601851) complex (PubMed: 21236481). This antibody can recognize CRY1 and CRY2 due to the high homology.

Protocols

Product Specific Protocols
WB protocol for Cryptochrome 1 antibody 13474-1-APDownload protocol
IHC protocol for Cryptochrome 1 antibody 13474-1-APDownload protocol
IF protocol for Cryptochrome 1 antibody 13474-1-APDownload protocol
FC protocol for Cryptochrome 1 antibody 13474-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Cell

Circadian clocks are modulated by compartmentalized oscillating translation

Authors - Yanrong Zhuang
View Article
WB

Sci Total Environ

Metastatic effects of perfluorooctanoic acid (PFOA) on Drosophila melanogaster with metabolic reprogramming and dysrhythmia in a multigenerational exposure scenario

Authors - Fangnon Firmin Fangninou
View Article
mouseWB

Sci Total Environ

Maternal circadian disruption before pregnancy impairs the ovarian function of female offspring in mice

Authors - Yajie Guan
View Article
humanIF

Theranostics

Development of human cartilage circadian rhythm in a stem cell-chondrogenesis model.

Authors - Mark A Naven
View Article
humanWB

EMBO Rep

Circadian networks in human embryonic stem cell-derived cardiomyocytes.

Authors - Pieterjan Dierickx
View Article
humanWB

Stem Cell Reports

SCA1(+) Cells from the Heart Possess a Molecular Circadian Clock and Display Circadian Oscillations in Cellular Functions.

Authors - Bastiaan C Du Pré
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Priya (Verified Customer) (01-11-2023)

I have used this for Human cardiomyocytes cell line and mice skin, and liver tissues

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Cardiomyoctyes, Keratinocytes, Liver and skin
FH

Iru (Verified Customer) (06-26-2020)

It works fine in 1:1000.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Fallopian tube epithelial cells