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CRTAP Polyclonal antibody

CRTAP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15724-1-AP

Synonyms

cartilage associated protein, CASP, CRTAP, LEPREL3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CRTAP Polyclonal antibody (15724-1-AP)

    WB analysis using 15724-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using CRTAP Polyclonal antibody (15724-1-AP)

    WB analysis of NIH/3T3 using 15724-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of ROS1728 cells using CRTAP Polyclonal antibody (15724-1-AP)

    WB analysis of ROS1728 using 15724-1-AP

    ROS1728 cells were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using CRTAP Polyclonal antibody (15724-1-AP)

    WB analysis of HT-1080 using 15724-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CRTAP Polyclonal antibody (15724-1-AP)

    WB analysis using 15724-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CRTAP Polyclonal antibody (15724-1-AP)

    WB analysis of HeLa using 15724-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CRTAP Polyclonal antibody (15724-1-AP)

    WB analysis of HEK-293 using 15724-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human colon tissue using CRTAP Polyclonal antibody (15724-1-AP)

    WB analysis of human colon using 15724-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human stomach tissue using CRTAP Polyclonal antibody (15724-1-AP)

    WB analysis of human stomach using 15724-1-AP

    human stomach tissue were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using CRTAP Polyclonal antibody (15724-1-AP)

    IP experiment of HEK-293 using 15724-1-AP

    IP result of anti-CRTAP (IP:15724-1-AP, 4ug; Detection:15724-1-AP 1:500) with HEK-293 cells lysate 3000 ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CRTAP Polyclonal antibody (15724-1-AP)

    IHC staining of human kidney using 15724-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15724-1-AP (CRTAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CRTAP Polyclonal antibody (15724-1-AP)

    IHC staining of human kidney using 15724-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15724-1-AP (CRTAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CRTAP Polyclonal antibody (15724-1-AP)

    IF Staining of HeLa using 15724-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CRTAP antibody (15724-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). F-actin was stained using CL594-phalloidin (red) and DNA was stained by DAPI (blue).

Tested Applications

Positive WB detected inHT-1080 cells, human stomach tissue, NIH/3T3 cells, ROS1728 cells, HEK-293 cells, HeLa cells, human colon tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

15724-1-AP targets CRTAP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRTAP fusion protein Ag8459
Full Name cartilage associated protein
Calculated Molecular Weight 46.5 kDa
Observed Molecular Weight 43-47 kDa
GenBank Accession NumberBC008745
Gene Symbol CRTAP
Gene ID (NCBI) 10491
RRIDAB_2261089
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CRTAP antibody 15724-1-APDownload protocol
IHC protocol for CRTAP antibody 15724-1-APDownload protocol
IF protocol for CRTAP antibody 15724-1-APDownload protocol
IP protocol for CRTAP antibody 15724-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Med Oncol

Data-independent acquisition (DIA) mass spectrometry reveals related proteins involved in the occurrence of early intestinal-type gastric cancer

Authors - Liangshun Zhang
View Article