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  • Featured Product
  • KD/KO Validated

CROP Polyclonal antibody

CROP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14504-1-AP

Synonyms

CREAP-1, CREAP1, CREAP 1, CRE associated protein 1, Cisplatin resistance-associated-overexpressed protein

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CROP Polyclonal antibody (14504-1-AP)

    WB analysis of Jurkat using 14504-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CROP Polyclonal antibody (14504-1-AP)

    WB analysis of HepG2 using 14504-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CROP Polyclonal antibody (14504-1-AP)

    WB analysis of mouse brain using 14504-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CROP Polyclonal antibody (14504-1-AP)

    WB analysis of HepG2 using 14504-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CROP Polyclonal antibody (14504-1-AP)

    WB analysis of Jurkat using 14504-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using CROP Polyclonal antibody (14504-1-AP)

    IP experiment of HepG2 using 14504-1-AP

    IP result of anti-CROP (IP:14504-1-AP, 4ug; Detection:14504-1-AP 1:500) with HepG2 cells lysate 3600ug.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using CROP Polyclonal antibody (14504-1-AP)

    IHC staining of human ovary cancer using 14504-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 14504-1-AP (CROP antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tissue using CROP Polyclonal antibody (14504-1-AP)

    IHC staining of human ovary using 14504-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using CROP Polyclonal antibody (14504-1-AP)

    IHC staining of human ovary using 14504-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CROP Polyclonal antibody (14504-1-AP)

    IHC staining of human pancreas cancer using 14504-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CROP Polyclonal antibody (14504-1-AP)

    IHC staining of human pancreas cancer using 14504-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CROP Polyclonal antibody (14504-1-AP)

    IF Staining of HepG2 using 14504-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CROP antibody (14504-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inJurkat cells, HepG2 cells, mouse brain tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman ovary cancer tissue, human ovary tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14504-1-AP targets CROP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CROP fusion protein Ag5961
Full Name cisplatin resistance-associated overexpressed protein
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 55-58 kDa
GenBank Accession NumberBC056409
Gene Symbol CROP
Gene ID (NCBI) 51747
RRIDAB_2229967
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CROP, a putative SR protein, contain cystine/histidine motifs and leucine zipper-like repeats in the N-terminal half and consists mostly of charged and polar amino acids in the C-terminal half. CROP has a high expression in heart, brain, pancreas, thymus, and weak in lung, live and kidney. As the human homologue of yeast Luc7p, CROP is supported to be participated in 5'-splice site recognize and is essential for vegetative growth. This is a rabbit polyclonal antibody raised against part of N-terminus of CROP of human origin, and can recognize a ~58kDa band of CROP.

Protocols

Product Specific Protocols
WB protocol for CROP antibody 14504-1-APDownload protocol
IHC protocol for CROP antibody 14504-1-APDownload protocol
IF protocol for CROP antibody 14504-1-APDownload protocol
IP protocol for CROP antibody 14504-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS Genet

Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.

Authors - Emily M Schleicher
  • KD Validated
View Article
humanWB

Cell Rep

Functional analyses of human LUC7-like proteins involved in splicing regulation and myeloid neoplasms.

Authors - Noah J Daniels
  • KD Validated
View Article
humanWB,IP

Cell Insight

LUC7L3 is a downstream factor of SRSF1 and prevents genomic instability

Authors - Xiaqing Zhang
View Article
humanWB

Curr Issues Mol Biol

Proteomics Identifies LUC7L3 as a Prognostic Biomarker for Hepatocellular Carcinoma

Authors - Yushan Hou
View Article
mouseIHC

Inorg Chem

Studies of Anticancer Activities In Vitro and In Vivo for Butyltin(IV)-Iridium(III) Imidazole-Phenanthroline Complexes with Aggregation-Induced Emission Properties

Authors - Yiwei Sun
View Article