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Carboxypeptidase A6 Polyclonal antibody

Carboxypeptidase A6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13604-1-AP

Synonyms

EC:3.4.17.-, CPA6, Carboxypeptidase B

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HepG2 cells using Carboxypeptidase A6 Polyclonal antibody (13604-1-AP)

    WB analysis of HepG2 using 13604-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13604-1-AP (Carboxypeptidase A6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using Carboxypeptidase A6 Polyclonal antibody (13604-1-AP)

    WB analysis of mouse brain using 13604-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13604-1-AP (Carboxypeptidase A6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using Carboxypeptidase A6 Polyclonal antibody (13604-1-AP)

    WB analysis of HepG2 using 13604-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13604-1-AP (Carboxypeptidase A6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using Carboxypeptidase A6 Polyclonal antibody (13604-1-AP)

    WB analysis of mouse brain using 13604-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13604-1-AP (Carboxypeptidase A6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Carboxypeptidase A6 Polyclonal antibody (13604-1-AP)

    IHC staining of human gliomas using 13604-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13604-1-AP (Carboxypeptidase A6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Carboxypeptidase A6 Polyclonal antibody (13604-1-AP)

    IHC staining of human gliomas using 13604-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13604-1-AP (Carboxypeptidase A6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Carboxypeptidase A6 Polyclonal antibody (13604-1-AP)

    IF Staining of HepG2 using 13604-1-AP

    Immunofluorescent analysis of HepG2 cells using 13604-1-AP (Carboxypeptidase A6 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, mouse brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13604-1-AP targets Carboxypeptidase A6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Carboxypeptidase A6 fusion protein Ag4466
Full Name carboxypeptidase A6
Calculated Molecular Weight 437 aa, 51 kDa
Observed Molecular Weight 65 kDa, 51 kDa
GenBank Accession NumberBC033684
Gene Symbol CPA6
Gene ID (NCBI) 57094
RRIDAB_2261427
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Carboxypeptidase A6 (CPA6) is an extracellular matrixbound metallocarboxypeptidase (CP) that has been implicated in Duane syndrome, a neurodevelopmental disorder in which the lateral rectus extraocularmuscle is not properly innervated.Consistent with a role inDuane syndrome, CPA6 is expressed in a number of chondrocytic and nervous tissues during embryogenesis.It has three isoforms.The product recognizes the C-terminal of this protein.

Protocols

Product Specific Protocols
WB protocol for Carboxypeptidase A6 antibody 13604-1-APDownload protocol
IHC protocol for Carboxypeptidase A6 antibody 13604-1-APDownload protocol
IF protocol for Carboxypeptidase A6 antibody 13604-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Arch Biochem Biophys

Carboxypeptidase A6 suppresses the proliferation and invasion of colorectal cancer cells and is negatively regulated by miR-96-3p

Authors - Xianren Wang
View Article