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CORO1A Polyclonal antibody

CORO1A Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IF-Fro, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 17760-1-AP

Synonyms

Coronin 1A, CORO1, Clipin-A, CLIPINA, Clipin A

Validation Data Gallery

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  • Western Blot (WB) analysis of rat brain tissue using CORO1A Polyclonal antibody (17760-1-AP)

    WB analysis of rat brain using 17760-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using CORO1A Polyclonal antibody (17760-1-AP)

    WB analysis of mouse spleen using 17760-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CORO1A Polyclonal antibody (17760-1-AP)

    WB analysis of Jurkat using 17760-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using CORO1A Polyclonal antibody (17760-1-AP)

    WB analysis of HL-60 using 17760-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat spleen tissue using CORO1A Polyclonal antibody (17760-1-AP)

    WB analysis of rat spleen using 17760-1-AP

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 17760-1-AP (CORO1A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using CORO1A Polyclonal antibody (17760-1-AP)

    IP experiment of mouse brain using 17760-1-AP

    IP result of anti-CORO1A (IP:17760-1-AP, 4ug; Detection:17760-1-AP 1:3000) with mouse brain tissue lysate 1840 ug.

  • Immunoprecipitation (IP) experiment of mouse spleen tissue using CORO1A Polyclonal antibody (17760-1-AP)

    IP experiment of mouse spleen using 17760-1-AP

    IP result of anti-CORO1A (IP:17760-1-AP, 4ug; Detection:17760-1-AP 1:1000) with mouse spleen tissue lysate 4800ug.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CORO1A Polyclonal antibody (17760-1-AP)

    IHC staining of mouse spleen using 17760-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 17760-1-AP (CORO1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CORO1A Polyclonal antibody (17760-1-AP)

    IHC staining of mouse spleen using 17760-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 17760-1-AP (CORO1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat spleen tissue using CORO1A Polyclonal antibody (17760-1-AP)

    IHC staining of rat spleen using 17760-1-AP

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 17760-1-AP (CORO1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat spleen tissue using CORO1A Polyclonal antibody (17760-1-AP)

    IHC staining of rat spleen using 17760-1-AP

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 17760-1-AP (CORO1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CORO1A Polyclonal antibody (17760-1-AP)

    IF Staining of mouse spleen using 17760-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CORO1A antibody (17760-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using CORO1A Polyclonal antibody (17760-1-AP)

    IF Staining of mouse colon using 17760-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CORO1A antibody (17760-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CORO1A Polyclonal antibody (17760-1-AP)

    IF Staining of mouse spleen using 17760-1-AP

    Immunofluorescent analysis of frozen OCT-embedded mouse spleen tissue using CORO1A antibody (17760-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of Jurkat cells using CORO1A Polyclonal antibody (17760-1-AP)

    FC experiment of Jurkat using 17760-1-AP

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human CORO1A (17760-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inmouse spleen tissue, HL-60 cells, Jurkat cells, rat brain tissue, rat spleen tissue
Positive IP detected inmouse brain tissue, mouse spleen tissue
Positive IHC detected inrat spleen tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue, mouse colon tissue
Positive IF-Fro detected inmouse spleen tissue
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)-FROIF-FRO : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

17760-1-AP targets CORO1A in WB, IHC, IF-P, IF-Fro, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CORO1A fusion protein Ag12139
Full Name coronin, actin binding protein, 1A
Calculated Molecular Weight 461 aa, 51 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC110374
Gene Symbol CORO1A
Gene ID (NCBI) 11151
RRIDAB_2082070
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CORO1A also known as TACO, belongs to the WD repeat coronin family. CORO1A is recruited to phagosomes and actively retained by surviving mycobacteria and is usually released before phagosome fusion with or maturation into lysosomes. CORO1A is expressed in the brain, thymus, spleen, bone marrow, and lymph nodes. Mutations in CORO1A cause Immunodeficiency 8 with lymphoproliferation (IMD8)(PMID: 10338208).

Protocols

Product Specific Protocols
WB protocol for CORO1A antibody 17760-1-APDownload protocol
IHC protocol for CORO1A antibody 17760-1-APDownload protocol
IF protocol for CORO1A antibody 17760-1-APDownload protocol
IP protocol for CORO1A antibody 17760-1-APDownload protocol
FC protocol for CORO1A antibody 17760-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Br J Pharmacol

PMID: 27128203

Authors - Xianrong Zhang
View Article
humanWB

Int Immunopharmacol

Carbamazepine-modified HLA-A*24:02-bound peptidome: Implication of CORO1A in skin rash.

Authors - Fuli Min
View Article
humanWB

Front Med (Lausanne)

MCC Regulator of WNT Signaling Pathway (MCC) Is a Podocyte Essential Gene.

Authors - Hui Song
View Article
ratWB

PLoS One

Repetitive Transcranial Magnetic Stimulation Promotes Neural Stem Cell Proliferation via the Regulation of MiR-25 in a Rat Model of Focal Cerebral Ischemia.

Authors - Feng Guo
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

lucie (Verified Customer) (06-25-2024)

Anti-CORO1A working in immunofluorescence on neutrophils fixed with PFA 2%, at a dilution of 1/200 in BSA 1%.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/200
  • Cell Tissue Type: Neutrophils