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COMMD8 Polyclonal antibody

COMMD8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25237-1-AP

Synonyms

MDS022, COMMD 8, COMM domain-containing protein 8

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using COMMD8 Polyclonal antibody (25237-1-AP)

    WB analysis of HEK-293 using 25237-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25237-1-AP (COMMD8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using COMMD8 Polyclonal antibody (25237-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 25237-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25237-1-AP (COMMD8 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using COMMD8 Polyclonal antibody (25237-1-AP)

    IF Staining of HEK-293 using 25237-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using COMMD8 antibody (25237-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Tested Applications

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

25237-1-AP targets COMMD8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COMMD8 fusion protein Ag18712
Full Name COMM domain containing 8
Calculated Molecular Weight 183 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC008371
Gene Symbol COMMD8
Gene ID (NCBI) 54951
RRIDAB_2879980
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for COMMD8 antibody 25237-1-APDownload protocol
IHC protocol for COMMD8 antibody 25237-1-APDownload protocol
IF protocol for COMMD8 antibody 25237-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

Structure of the endosomal Commander complex linked to Ritscher-Schinzel syndrome

Authors - Michael D Healy
View Article