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  • Featured Product
  • KD/KO Validated

COMMD5 Polyclonal antibody

COMMD5 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10393-1-AP

Synonyms

Hypertension-related calcium-regulated gene protein, HT002, HCaRG, COMM domain-containing protein 5, COMM domain containing 5

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using COMMD5 Polyclonal antibody (10393-1-AP)

    WB analysis of mouse kidney using 10393-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using COMMD5 Polyclonal antibody (10393-1-AP)

    WB analysis of human placenta using 10393-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using COMMD5 Polyclonal antibody (10393-1-AP)

    WB analysis of mouse heart using 10393-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using COMMD5 Polyclonal antibody (10393-1-AP)

    WB analysis of HEK-293 using 10393-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse stomach tissue using COMMD5 Polyclonal antibody (10393-1-AP)

    WB analysis of mouse stomach using 10393-1-AP

    mouse stomach tissue were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using COMMD5 Polyclonal antibody (10393-1-AP)

    WB analysis of MCF-7 using 10393-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using COMMD5 Polyclonal antibody (10393-1-AP)

    WB analysis of human heart using 10393-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human stomach tissue using COMMD5 Polyclonal antibody (10393-1-AP)

    WB analysis of human stomach using 10393-1-AP

    human stomach tissue were subjected to SDS PAGE followed by western blot with 10393-1-AP (COMMD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using COMMD5 Polyclonal antibody (10393-1-AP)

    IP experiment of mouse heart using 10393-1-AP

    IP result of anti-COMMD5 (IP:10393-1-AP, 4ug; Detection:10393-1-AP 1:500) with mouse heart tissue lysate 3200ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using COMMD5 Polyclonal antibody (10393-1-AP)

    IF Staining of HeLa using 10393-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10393-1-AP (COMMD5 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse kidney tissue, human heart tissue, human stomach tissue, mouse stomach tissue, mouse heart tissue, HEK-293 cells, MCF-7 cells, human placenta tissue
Positive IP detected inmouse heart tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10393-1-AP targets COMMD5 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COMMD5 fusion protein Ag0592
Full Name COMM domain containing 5
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC003055
Gene Symbol COMMD5
Gene ID (NCBI) 28991
RRIDAB_2083555
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COMM domain containing 5 (COMMD5, synonyms: HCARG, HT002) is a hypertension-related calcium-regulated gene. COMMD5 is negatively regulated by extracellular calcium concentration, and its basal mRNA levels were higher in hypertensive animals. Tissue distribution of COMMD5 shows a preponderance in the heart, stomach, jejunum, kidney (tubular fraction), liver, and adrenal gland (mainly in the medulla). COMMD5 mRNA is significantly more expressed in adult than in fetal organs, and its levels are decreased in tumors and cancerous cell lines. COMMD5 protein has no transmembrane domain, but contains 67% -helix content, a calcium-binding site, four putative "leucine zipper" motifs, and a nuclear receptor-binding domain. At the subcellular level, COMMD5 shows a nuclear localization.

Protocols

Product Specific Protocols
WB protocol for COMMD5 antibody 10393-1-APDownload protocol
IF protocol for COMMD5 antibody 10393-1-APDownload protocol
IP protocol for COMMD5 antibody 10393-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

J Am Soc Nephrol

HCaRG accelerates tubular repair after ischemic kidney injury.

Authors - Hiroyuki Matsuda
View Article
humanWB,IF,CoIP

Cell Rep

COMMD5/HCaRG Hooks Endosomes on Cytoskeleton and Coordinates EGFR Trafficking.

Authors - Carole G Campion
  • KD Validated
View Article
humanIF

Elife

Lipid kinases VPS34 and PIKfyve coordinate a phosphoinositide cascade to regulate retriever-mediated recycling on endosomes.

Authors - Sai Srinivas Panapakkam Giridharan
View Article
humanWB

Mol Metab

Genome-wide CRISPR screen identified a role for commander complex mediated ITGB1 recycling in basal insulin secretion.

Authors - Liu Yang
View Article
humanIHC

Oncol Rep

PMID: 18949406

Authors - Chen Bai-Li BL
View Article
humanIHC

Anticancer Res

COMMD5 Inhibits Malignant Behavior of Renal Cancer Cells.

Authors - Jin Ikeda
View Article