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Collagen Type VIII Polyclonal antibody
Collagen Type VIII Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 17251-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | A375 cells, HeLa cells, HepG2 cells, COLO 320 cells, mouse kidney tissue, mouse spleen tissue, rat kidney tissue |
| Positive IP detected in | HepG2 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
| IHC | See 2 publications below |
| IF | See 3 publications below |
Product Information
The immunogen of 17251-1-AP is Collagen Type VIII Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Collagen Type VIII fusion protein Ag10607 |
| Full Name | collagen, type VIII, alpha 1 |
| Calculated Molecular Weight | 744 aa, 73 kDa |
| Observed Molecular Weight | 73 kDa |
| GenBank Accession Number | BC013581 |
| Gene Symbol | COL8A1 |
| Gene ID (NCBI) | 1295 |
| RRID | AB_10696173 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Collagen Type VIII antibody 17251-1-AP | Download protocol |
| IF protocol for Collagen Type VIII antibody 17251-1-AP | Download protocol |
| IP protocol for Collagen Type VIII antibody 17251-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Bioeng Biotechnol Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts. | ||
Front Cell Dev Biol Platelet-Derived Extracellular Vesicles Increase Col8a1 Secretion and Vascular Stiffness in Intimal Injury.
| ||
FASEB J Silencing of METTL3 attenuates cardiac fibrosis induced by myocardial infarction via inhibiting the activation of cardiac fibroblasts. | ||
Biomed Pharmacother Compound Danshen Dripping Pill inhibits doxorubicin or isoproterenol-induced cardiotoxicity. | ||
Dis Model Mech Upregulation of distinct collagen transcripts in post-surgery scar tissue: a study of conjunctival fibrosis. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Kenzo (Verified Customer) (11-11-2023) | This antibody worked well for mouse eye cryosections.
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