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Collagen Type II Polyclonal antibody

Collagen Type II Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15943-1-AP

Synonyms

Alpha 1 type II collagen, ANFH, AOM, COL11A3, COL2A1, Collagen alpha 1(II) chain, Collagen Type II, collagen, type II, alpha 1, SEDC

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human lung tissue using Collagen Type II Polyclonal antibody (15943-1-AP)

    IHC staining of human lung using 15943-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15943-1-AP (Collagen Type II antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using Collagen Type II Polyclonal antibody (15943-1-AP)

    IHC staining of human lung using 15943-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15943-1-AP (Collagen Type II antibody at dilution of 1:50 (under 40x lens).

Tested Applications

Positive IHC detected inhuman lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 15943-1-AP is Collagen Type II Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, pig, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Collagen Type II fusion protein Ag8416
Full Name collagen, type II, alpha 1
Calculated Molecular Weight 134 kDa
GenBank Accession NumberBC007252
Gene Symbol COL2A1
Gene ID (NCBI) 1280
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for Collagen Type II antibody 15943-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

Autophagy

HIF1A Alleviates compression-induced apoptosis of nucleus pulposus derived stem cells via upregulating autophagy.

Authors - Ruijun He
View Article
humanIHC

J Tissue Eng

High BMPR2 expression leads to enhanced SMAD1/5/8 signalling and GDF6 responsiveness in human adipose-derived stem cells: implications for stem cell therapies for intervertebral disc degeneration.

Authors - Tom Hodgkinson
View Article
humanIHC

Redox Biol

Advanced oxidation protein products increase TNF-α and IL-1β expression in chondrocytes via NADPH oxidase 4 and accelerate cartilage degeneration in osteoarthritis progression.

Authors - Cong-Rui Liao
View Article
human

Oxid Med Cell Longev

KLF2 Protects against Osteoarthritis by Repressing Oxidative Response through Activation of Nrf2/ARE Signaling In Vitro and In Vivo.

Authors - Xiang Gao
View Article
mouseIF

Oxid Med Cell Longev

Bone Marrow Mesenchymal Stem Cell-Derived Extracellular Vesicles Carrying circ_0050205 Attenuate Intervertebral Disc Degeneration.

Authors - Xiao-Jun Yu
View Article

Int J Mol Sci

Melatonin Promotes Antler Growth by Accelerating MT1-Mediated Mesenchymal Cell Differentiation and Inhibiting VEGF-Induced Degeneration of Chondrocytes.

Authors - Xuyang Sun
View Article