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  • Featured Product
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CNN2 Monoclonal antibody

CNN2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G10F5

Cat no : 66244-1-Ig

Synonyms

calponin 2, Calponin H2, smooth muscle, CNN2, Neutral calponin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CNN2 Monoclonal antibody (66244-1-Ig)

    WB analysis using 66244-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66244-1-Ig (CNN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of HeLa cells using CNN2 Monoclonal antibody (66244-1-Ig)

    WB analysis of HeLa using 66244-1-Ig

    WB result of CNN2 antibody (66244-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNN2 transfected HeLa cells.

  • Western Blot (WB) analysis of A431 cells using CNN2 Monoclonal antibody (66244-1-Ig)

    WB analysis of A431 using 66244-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 66244-1-Ig (CNN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CNN2 Monoclonal antibody (66244-1-Ig)

    WB analysis of HeLa using 66244-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66244-1-Ig (CNN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig stomach tissue using CNN2 Monoclonal antibody (66244-1-Ig)

    WB analysis of pig stomach using 66244-1-Ig

    pig stomach tissue were subjected to SDS PAGE followed by western blot with 66244-1-Ig (CNN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using CNN2 Monoclonal antibody (66244-1-Ig)

    WB analysis of HT-1080 using 66244-1-Ig

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 66244-1-Ig (CNN2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast hyperplasia tissue using CNN2 Monoclonal antibody (66244-1-Ig)

    IHC staining of human breast hyperplasia using 66244-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66244-1-Ig (CNN2 Antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast hyperplasia tissue using CNN2 Monoclonal antibody (66244-1-Ig)

    IHC staining of human breast hyperplasia using 66244-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 66244-1-Ig (CNN2 Antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHepG2 cells, A431 cells, HeLa cells, pig stomach tissue, HT-1080 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman breast hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66244-1-Ig targets CNN2 in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CNN2 fusion protein Ag23594
Full Name calponin 2
Calculated Molecular Weight 309 aa, 34 kDa
Observed Molecular Weight 34-36 kDa
GenBank Accession NumberBC141818
Gene Symbol CNN2
Gene ID (NCBI) 1265
RRIDAB_2881633
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). Calponin 1 and calponin 2 are predominately expressed in smooth muscle cells and cardiac muscle cells, respectively. Calponin 3 is highly expressed in many tissues including articular cartilage and brain.

Protocols

Product Specific Protocols
WB protocol for CNN2 antibody 66244-1-IgDownload protocol
IHC protocol for CNN2 antibody 66244-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols