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CNGA3 Polyclonal antibody

CNGA3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21657-1-AP

Synonyms

ACHM2, CCNC1, CCNCa, CCNCalpha, CNCG3, CNG 3, CNG channel alpha 3, CNG3, CNGA3

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using CNGA3 Polyclonal antibody (21657-1-AP)

    WB analysis of HEK-293 using 21657-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21657-1-AP (CNGA3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CNGA3 Polyclonal antibody (21657-1-AP)

    WB analysis of HEK-293 using 21657-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21657-1-AP (CNGA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using CNGA3 Polyclonal antibody (21657-1-AP)

    IP experiment of mouse brain using 21657-1-AP

    IP result of anti-CNGA3 (IP:21657-1-AP, 4ug; Detection:21657-1-AP 1:300) with mouse brain tissue lysate 3000ug.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using CNGA3 Polyclonal antibody (21657-1-AP)

    IHC staining of mouse eye using 21657-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 21657-1-AP (CNGA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using CNGA3 Polyclonal antibody (21657-1-AP)

    IHC staining of mouse eye using 21657-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 21657-1-AP (CNGA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21657-1-AP is CNGA3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNGA3 fusion protein Ag16385
Full Name cyclic nucleotide gated channel alpha 3
Calculated Molecular Weight 694 aa, 79 kDa
Observed Molecular Weight 98 kDa
GenBank Accession NumberBC096298
Gene Symbol CNGA3
Gene ID (NCBI) 1261
RRIDAB_10734591
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CNGA3 is a member of the cyclic nucleotide-gated cation channel protein family which is required for normal vision and olfactory signal transduction. Mutations in CNGA3 gene are associated with achromatopsia (rod monochromacy) and color blindness. Three alternatively spliced transcripts encoding different isoforms have been described. This antibody detects CNGA3 with an apparent molecular weight of 98 kDa which has been reported (PMID: 20378608).

Protocols

Product Specific Protocols
WB protocol for CNGA3 antibody 21657-1-APDownload protocol
IHC protocol for CNGA3 antibody 21657-1-APDownload protocol
IP protocol for CNGA3 antibody 21657-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols