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Calmegin Polyclonal antibody

Calmegin Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12629-1-AP

Synonyms

calmegin, CLGN

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using Calmegin Polyclonal antibody (12629-1-AP)

    WB analysis of mouse testis using 12629-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12629-1-AP (Calmegin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using Calmegin Polyclonal antibody (12629-1-AP)

    WB analysis of mouse lung using 12629-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 12629-1-AP (Calmegin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using Calmegin Polyclonal antibody (12629-1-AP)

    WB analysis of human brain using 12629-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12629-1-AP (Calmegin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human lung tissue using Calmegin Polyclonal antibody (12629-1-AP)

    WB analysis of human lung using 12629-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 12629-1-AP (Calmegin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using Calmegin Polyclonal antibody (12629-1-AP)

    WB analysis of mouse brain using 12629-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12629-1-AP (Calmegin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using Calmegin Polyclonal antibody (12629-1-AP)

    IP experiment of mouse testis using 12629-1-AP

    IP result of anti-Calmegin (IP:12629-1-AP, 4ug; Detection:12629-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using Calmegin Polyclonal antibody (12629-1-AP)

    IHC staining of human testis using 12629-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12629-1-AP (Calmegin antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using Calmegin Polyclonal antibody (12629-1-AP)

    IHC staining of human testis using 12629-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12629-1-AP (Calmegin antibody at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse testis tissue, human brain tissue, human lung tissue, mouse brain tissue, mouse lung tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12629-1-AP targets Calmegin in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Calmegin fusion protein Ag3322
Full Name calmegin
Calculated Molecular Weight 610 aa, 70 kDa
Observed Molecular Weight 93 kDa
GenBank Accession NumberBC028357
Gene Symbol Calmegin
Gene ID (NCBI) 1047
RRIDAB_2276364
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calmegin, belonging to the calreticulin family, is a testis-specific molecular chaperon of the endoplasmic reticulum playing a key role in spermatogenesis. Western blot analysis of mouse testis showed a single band with an apparent molecular mass of 93 kDa.

Protocols

Product Specific Protocols
WB protocol for Calmegin antibody 12629-1-APDownload protocol
IHC protocol for Calmegin antibody 12629-1-APDownload protocol
IP protocol for Calmegin antibody 12629-1-APDownload protocol
FC protocol for Calmegin antibody 12629-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell Proteomics

Integrative proteomic and transcriptomic analyses reveal multiple post-transcriptional regulatory mechanisms of mouse spermatogenesis.

Authors - Haiyun Gan
View Article
mouseIF

Sci Rep

Transcription Factor RFX2 Is a Key Regulator of Mouse Spermiogenesis.

Authors - Yujian Wu
View Article