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CKAP4 Polyclonal antibody

CKAP4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16686-1-AP

Synonyms

63 kDa membrane protein, CKAP4, CLIMP 63, ERGIC 63, p63

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CKAP4 Polyclonal antibody (16686-1-AP)

    WB analysis using 16686-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16686-1-AP (CKAP4 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CKAP4 Polyclonal antibody (16686-1-AP)

    WB analysis using 16686-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16686-1-AP (CKAP4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat kidney tissue using CKAP4 Polyclonal antibody (16686-1-AP)

    WB analysis of rat kidney using 16686-1-AP

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 16686-1-AP (CKAP4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using CKAP4 Polyclonal antibody (16686-1-AP)

    IP experiment of HeLa using 16686-1-AP

    IP result of anti-CKAP4 (IP:16686-1-AP, 4ug; Detection:16686-1-AP 1:1000) with HeLa cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CKAP4 Polyclonal antibody (16686-1-AP)

    IHC staining of human kidney using 16686-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16686-1-AP (CKAP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CKAP4 Polyclonal antibody (16686-1-AP)

    IHC staining of human kidney using 16686-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16686-1-AP (CKAP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using CKAP4 Polyclonal antibody (16686-1-AP)

    IHC staining of human liver using 16686-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver slide using 16686-1-AP (CKAP4 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using CKAP4 Polyclonal antibody (16686-1-AP)

    IHC staining of human liver using 16686-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver slide using 16686-1-AP (CKAP4 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CKAP4 Polyclonal antibody (16686-1-AP)

    IF Staining of HepG2 using 16686-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CKAP4 antibody (16686-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CKAP4 Polyclonal antibody (16686-1-AP)

    IF Staining of HepG2 using 16686-1-AP

    Immunofluorescent analysis of HepG2 cells, using CKAP4 antibody 16686-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CKAP4 Polyclonal antibody (16686-1-AP)

    IF Staining of HepG2 using 16686-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CKAP4 antibody (16686-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inA431 cells, rat kidney tissue, HeLa cells, HepG2 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16686-1-AP targets CKAP4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CKAP4 fusion protein Ag10019
Full Name cytoskeleton-associated protein 4
Calculated Molecular Weight 602 aa, 66 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC082972
Gene Symbol CKAP4
Gene ID (NCBI) 10970
RRIDAB_2276275
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CKAP4, also named as Climp-63, is a 63 kDa cytoskeleton-linking membrane protein. It is the partner of triadin. CKAP4 is responsible for this association of triads and microtubules.

Protocols

Product Specific Protocols
WB protocol for CKAP4 antibody 16686-1-APDownload protocol
IHC protocol for CKAP4 antibody 16686-1-APDownload protocol
IF protocol for CKAP4 antibody 16686-1-APDownload protocol
IP protocol for CKAP4 antibody 16686-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

Regulation of endoplasmic reticulum turnover by selective autophagy.

Authors - Aliaksandr Khaminets
View Article
ratWB

Cell Metab

Palmitoylation couples insulin hypersecretion with β cell failure in diabetes

Authors - Guifang Dong
View Article
humanWB

Nat Cell Biol

Combinatorial selective ER-phagy remodels the ER during neurogenesis

Authors - Melissa J Hoyer
View Article
humanWB

Mol Cell

Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex

Authors - Michael L van de Weijer
View Article
humanWB

Mol Cell

Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex.

Authors - Michael L van de Weijer
View Article
humanIF

Dev Cell

Sestrin2 drives ER-phagy in response to protein misfolding

Authors - Chiara De Leonibus
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

WEI (Verified Customer) (06-28-2020)

Single strong band at correct size.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: Mouse Heart