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  • Featured Product
  • KD/KO Validated

CKAP2 Polyclonal antibody

CKAP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25486-1-AP

Synonyms

Tumor- and microtubule-associated protein, TMAP, se20-10, LB1, Cytoskeleton-associated protein 2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CKAP2 Polyclonal antibody (25486-1-AP)

    WB analysis of HeLa using 25486-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25486-1-AP (CKAP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CKAP2 Polyclonal antibody (25486-1-AP)

    WB analysis of HEK-293 using 25486-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25486-1-AP (CKAP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using CKAP2 Polyclonal antibody (25486-1-AP)

    WB analysis of PC-3 using 25486-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25486-1-AP (CKAP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SGC-7901 cells using CKAP2 Polyclonal antibody (25486-1-AP)

    WB analysis of SGC-7901 using 25486-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 25486-1-AP (CKAP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using CKAP2 Polyclonal antibody (25486-1-AP)

    IHC staining of human skin cancer using 25486-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 25486-1-AP (CKAP2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CKAP2 Polyclonal antibody (25486-1-AP)

    IF Staining of HeLa using 25486-1-AP

    Immunofluorescent analysis of HeLa cells using 25486-1-AP (CKAP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, PC-3 cells, SGC-7901 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25486-1-AP targets CKAP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CKAP2 fusion protein Ag21901
Full Name cytoskeleton associated protein 2
Calculated Molecular Weight 683 aa, 77 kDa
Observed Molecular Weight 75-85 kDa
GenBank Accession NumberBC136332
Gene Symbol CKAP2
Gene ID (NCBI) 26586
RRIDAB_2880100
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CKAP2 antibody 25486-1-APDownload protocol
IHC protocol for CKAP2 antibody 25486-1-APDownload protocol
IF protocol for CKAP2 antibody 25486-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Proc Natl Acad Sci U S A

The spindle protein CKAP2 regulates microtubule dynamics and ensures faithful chromosome segregation

Authors - Lia Mara Gomes Paim
View Article
humanWB

Oncol Rep

CKAP2 expression is associated with glioma tumor growth and acts as a prognostic factor in high‑grade glioma.

Authors - Kuanyu Wang
  • KD Validated
View Article
humanWB,IF

Cancers (Basel)

Knockdown of CKAP2 Inhibits Proliferation, Migration, and Aggregate Formation in Aggressive Breast Cancer

Authors - Alexsandro dos Santos
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Azriel (Verified Customer) (12-02-2019)

Worked great for IF of HT-1080 cells at a 1/400 dilution incubation overnight

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1/400
  • Cell Tissue Type: HT-1080 human fibrosarcoma cell line