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CITED2 Polyclonal antibody

CITED2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15021-1-AP

Synonyms

MSG-related protein 1, MSG related protein 1, MRG-1, MRG1, MRG 1

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using CITED2 Polyclonal antibody (15021-1-AP)

    WB analysis of SH-SY5Y using 15021-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15021-1-AP (CITED2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CITED2 Polyclonal antibody (15021-1-AP)

    WB analysis of mouse brain using 15021-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15021-1-AP (CITED2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CITED2 Polyclonal antibody (15021-1-AP)

    IF Staining of SH-SY5Y using 15021-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed SH-SY5Y cells using 15021-1-AP (CITED2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inSH-SY5Y cells, mouse brain tissue
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15021-1-AP targets CITED2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CITED2 fusion protein Ag7078
Full Name Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 36 kDa, 27 kDa
GenBank Accession NumberBC004377
Gene Symbol CITED2
Gene ID (NCBI) 10370
RRIDAB_2080545
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CITED2, also named as P35srj, is a cAMP-responsive element-binding protein (CBP)/p300-transactivator, with an ED(Glu/Asp)-rich C-terminal domain that acts as an important modulator in the development of the heart [PMID:10552932]. There are two isoforms of CITED2 protein and the calculated molecular weight of them are 24kDa and 28 kDa. The 28 kDa isoform having modification site may migrate to 35 kDa and 24 kDa isoform having modification site may migrate to 27 kDa (PMID: 23082118). It is proposed to be a negative regulator of HIF-1α through its competitive binding to the CH1 domain of CBP/p300, which mediated signaling and to a decrease in HIF-1-responsive genes such as VEGF [PMID:9887100]. In addition, it is also a co-activator of the transcriptional activity of the TFAP2 isoform, which is an extensively expressed nuclear protein with functions in embryogenesis, inflammation, and stress responses[PMID:22735262].

Protocols

Product Specific Protocols
WB protocol for CITED2 antibody 15021-1-APDownload protocol
IF protocol for CITED2 antibody 15021-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Toxicol Sci

The role of miR-182-5p in hepatocarcinogenesis of trichloroethylene in mice.

Authors - Yan Jiang
View Article