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CIP29 Polyclonal antibody

CIP29 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15798-1-AP

Synonyms

Proliferation-associated cytokine-inducible protein CIP29, Nuclear protein Hcc-1, HSPC316, HCC1, Cytokine-induced protein of 29 kDa

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CIP29 Polyclonal antibody (15798-1-AP)

    WB analysis using 15798-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15798-1-AP (CIP29 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using CIP29 Polyclonal antibody (15798-1-AP)

    WB analysis of Raji using 15798-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 15798-1-AP (CIP29 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using CIP29 Polyclonal antibody (15798-1-AP)

    WB analysis of rat liver using 15798-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 15798-1-AP (CIP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat pancreas tissue using CIP29 Polyclonal antibody (15798-1-AP)

    WB analysis of rat pancreas using 15798-1-AP

    rat pancreas tissue were subjected to SDS PAGE followed by western blot with 15798-1-AP (CIP29 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CIP29 Polyclonal antibody (15798-1-AP)

    IHC staining of human stomach cancer using 15798-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15798-1-AP (CIP29 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CIP29 Polyclonal antibody (15798-1-AP)

    IF Staining of MCF-7 using 15798-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CIP29 antibody (15798-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CIP29 Polyclonal antibody (15798-1-AP)

    IF Staining of MCF-7 using 15798-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CIP29 antibody (15798-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, Raji cells, rat pancreas tissue, HeLa cells, MCF-7 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1500-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15798-1-AP targets CIP29 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CIP29 fusion protein Ag8507
Full Name cytokine induced protein 29 kDa
Calculated Molecular Weight 210 aa, 24 kDa
Observed Molecular Weight 29-32 kDa
GenBank Accession NumberBC007099
Gene Symbol CIP29
Gene ID (NCBI) 84324
RRIDAB_1959356
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CIP29 antibody 15798-1-APDownload protocol
IHC protocol for CIP29 antibody 15798-1-APDownload protocol
IF protocol for CIP29 antibody 15798-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Tumour Biol

Depletion of Dicer promotes epithelial ovarian cancer progression by elevating PDIA3 expression.

Authors - Ying Zhu
View Article
humanWB,IF

PLoS Pathog

TREX (transcription/export)-NP complex exerts a dual effect on regulating polymerase activity and replication of influenza A virus

Authors - Lingcai Zhao
  • KD Validated
View Article