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CIB4 Polyclonal antibody

CIB4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18840-1-AP

Synonyms

CIB4, KIP4

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using CIB4 Polyclonal antibody (18840-1-AP)

    WB analysis of mouse lung using 18840-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 18840-1-AP (CIB4 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using CIB4 Polyclonal antibody (18840-1-AP)

    WB analysis of mouse lung using 18840-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 18840-1-AP (CIB4 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CIB4 Polyclonal antibody (18840-1-AP)

    IHC staining of human kidney using 18840-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18840-1-AP (CIB4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CIB4 Polyclonal antibody (18840-1-AP)

    IHC staining of human kidney using 18840-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18840-1-AP (CIB4 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18840-1-AP targets CIB4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name calcium and integrin binding family member 4
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC140923
Gene Symbol CIB4
Gene ID (NCBI) 130106
RRIDAB_10598172
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CIB4 antibody 18840-1-APDownload protocol
IHC protocol for CIB4 antibody 18840-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols