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CIAO1 Polyclonal antibody

CIAO1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10295-1-AP

Synonyms

WDR39, WD repeat-containing protein 39, Probable cytosolic iron-sulfur protein assembly protein CIAO1, CIA1

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using CIAO1 Polyclonal antibody (10295-1-AP)

    WB analysis of HepG2 using 10295-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10295-1-AP (CIAO1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using CIAO1 Polyclonal antibody (10295-1-AP)

    WB analysis of PC-3 using 10295-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 10295-1-AP (CIAO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using CIAO1 Polyclonal antibody (10295-1-AP)

    WB analysis of MCF-7 using 10295-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 10295-1-AP (CIAO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using CIAO1 Polyclonal antibody (10295-1-AP)

    IHC staining of human testis using 10295-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10295-1-AP (CIAO1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using CIAO1 Polyclonal antibody (10295-1-AP)

    IHC staining of human small intestine using 10295-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10295-1-AP (CIAO1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using CIAO1 Polyclonal antibody (10295-1-AP)

    IHC staining of human small intestine using 10295-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10295-1-AP (CIAO1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CIAO1 Polyclonal antibody (10295-1-AP)

    IHC staining of human testis using 10295-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10295-1-AP (CIAO1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CIAO1 Polyclonal antibody (10295-1-AP)

    IHC staining of human testis using 10295-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10295-1-AP (CIAO1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CIAO1 Polyclonal antibody (10295-1-AP)

    IHC staining of human testis using 10295-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10295-1-AP (CIAO1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CIAO1 Polyclonal antibody (10295-1-AP)

    IF Staining of HepG2 using 10295-1-AP

    Immunofluorescent analysis of HepG2 cells using 10295-1-AP (CIAO1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, PC-3 cells
Positive IHC detected inhuman small intestine tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10295-1-AP targets CIAO1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CIAO1 fusion protein Ag0349
Full Name cytosolic iron-sulfur protein assembly 1 homolog (S. cerevisiae)
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 48-55 kDa
GenBank Accession NumberBC001395
Gene Symbol CIAO1
Gene ID (NCBI) 9391
RRIDAB_2260509
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CIAO1, also named as CIA1 or WDR39, is a essential component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. CIAO1 seems to specifically modulate the transactivation activity of WT1 and may function to regulate the physiological functions of WT1 in cell growth and differentiation. It may also play a role in chromosome segregation. The predicted MW of this protein is 38 kDa. 10295-1-AP detected 48-55 kDa band because of phosphorylation. And 10295-1-AP do well in IHC and IF detection.

Protocols

Product Specific Protocols
WB protocol for CIAO1 antibody 10295-1-APDownload protocol
IHC protocol for CIAO1 antibody 10295-1-APDownload protocol
IF protocol for CIAO1 antibody 10295-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols