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CHST9 Polyclonal antibody

CHST9 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13135-1-AP

Synonyms

UNQ2549/PRO6175, N-acetylgalactosamine-4-O-sulfotransferase 2, GalNAc4ST-2, GalNAc-4-ST2, GalNAc-4-O-sulfotransferase 2

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using CHST9 Polyclonal antibody (13135-1-AP)

    WB analysis of HepG2 using 13135-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13135-1-AP (CHST9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using CHST9 Polyclonal antibody (13135-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 13135-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 13135-1-AP (CHST9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHepG2 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13135-1-AP targets CHST9 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHST9 fusion protein Ag3874
Full Name carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 9
Calculated Molecular Weight 438 aa, 52 kDa
Observed Molecular Weight 52 kDa, 42 kDa
GenBank Accession NumberBC025764
Gene Symbol CHST9
Gene ID (NCBI) 83539
RRIDAB_2079222
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CHST9 antibody 13135-1-APDownload protocol
IHC protocol for CHST9 antibody 13135-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols