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  • Featured Product
  • KD/KO Validated

CHST2 Polyclonal antibody

CHST2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26027-1-AP

Synonyms

C6ST, CHST2, GlcNAc6ST 1, GN6ST, Gn6ST 1, GST 2, GST2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using CHST2 Polyclonal antibody (26027-1-AP)

    WB analysis of mouse brain using 26027-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 26027-1-AP (CHST2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26027-1-AP targets CHST2 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHST2 fusion protein Ag23293
Full Name carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2
Observed Molecular Weight 50-52 kDa, 58-60 kDa
GenBank Accession NumberBC105010
Gene Symbol CHST2
Gene ID (NCBI) 9435
RRIDAB_2880342
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHST2 encodes N-Acetylglucosamine-6-sulfotransferase-1 (GlcNAc6ST-1), a Golgi-resident glycoprotein that is responsible for sulfation of the L-selectin ligand on endothelial cells. Two isoforms of CHST2 exist due to the alternative splicing. This antibody recognizes both short form and long form, which migrates around 50-52 kDa and 58-60 kDa, respectively.

Protocols

Product Specific Protocols
WB protocol for CHST2 antibody 26027-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

CHST2-mediated sulfation of MECA79 antigens is critical for breast cancer cell migration and metastasis

Authors - Dan Zhang
  • KD Validated
View Article