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- KD/KO Validated
Cingulin Polyclonal antibody
Cingulin Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 21369-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, mouse kidney tissue, mouse small intestine tissue, MCF-7 cells |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
| IHC | See 1 publications below |
| IF | See 5 publications below |
| IP | See 1 publications below |
Product Information
21369-1-AP targets Cingulin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Cingulin fusion protein Ag15843 |
| Full Name | cingulin |
| Calculated Molecular Weight | 1197 aa, 136 kDa |
| Observed Molecular Weight | 136-150 kDa |
| GenBank Accession Number | BC146657 |
| Gene Symbol | Cingulin |
| Gene ID (NCBI) | 57530 |
| RRID | AB_10733647 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cingulin is a component of the cytoplasmic plaque of tight junction, and is localized on the cytoplasmic face of tight junctions of polarized epithelia and some endothelia. Cingulin is a homodimer, each subunit containing a globular "head" domain, a coiled-coil "rod" domain and a small globular "tail" region. Cingulin can interact interact with ZO-1, ZO-2, ZO-3, actin, and myosin. It probably plays a role in the formation and regulation of the tight junction paracellular permeability barrier.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Cingulin antibody 21369-1-AP | Download protocol |
| IHC protocol for Cingulin antibody 21369-1-AP | Download protocol |
| IP protocol for Cingulin antibody 21369-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock. | ||
Nat Commun Cingulin and actin mediate midbody-dependent apical lumen formation during polarization of epithelial cells.
| ||
Mucosal Immunol Epithelial NELF guards intestinal barrier function to ameliorate colitis by maintaining junctional integrity. | ||
Cancers (Basel) miR-125b Promotes Colorectal Cancer Migration and Invasion by Dual-Targeting CFTR and CGN. | ||
Sci Rep Treatment with Ligilactobacillus murinus lowers blood pressure and intestinal permeability in spontaneously hypertensive rats | ||
Cell Signal Motif affinity and mass spectrometry proteomic approach for the discovery of cellular AMPK targets: Identification of mitochondrial fission factor as a new AMPK substrate. |
