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CES2 Polyclonal antibody
CES2 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 15378-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | BxPC-3 cells, human liver tissue, HuH-7 cells |
| Positive IHC detected in | mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
Product Information
15378-1-AP targets CES2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CES2 fusion protein Ag2218 |
| Full Name | carboxylesterase 2 (intestine, liver) |
| Calculated Molecular Weight | 62 kDa |
| Observed Molecular Weight | 60-70 kDa |
| GenBank Accession Number | BC032095 |
| Gene Symbol | CES2 |
| Gene ID (NCBI) | 8824 |
| RRID | AB_2077501 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CES2(Carboxylesterase 2) is also named as ICE and belongs to the type-B carboxylesterase/lipase family. Carboxylesterases (CESs) hydrolyze ester groups of drugs and toxins. CES2 specifically catalyzes the hydrolysis of the benzoyl group of cocaine and the acetyl groups of 4-methylumbelliferyl acetate, heroin, and 6-monoacetylmorphine. CES2 differs from another liver carboxylesterase, CES1 (114835), in drug ester substrate specificity. This protein has 2 isoforms produced by alternative splicing
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CES2 antibody 15378-1-AP | Download protocol |
| IHC protocol for CES2 antibody 15378-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Drug Metab Dispos Simultaneous absolute protein quantification of carboxylesterases 1 and 2 in human liver tissue fractions using liquid chromatography-tandem mass spectrometry. | ||
Mol Metab Carboxylesterase 2 induces mitochondrial dysfunction via disrupting lipid homeostasis in oral squamous cell carcinoma
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