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  • Featured Product
  • KD/KO Validated

CES1 Polyclonal antibody

CES1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16912-1-AP

Synonyms

ACAT, Brain carboxylesterase hBr1, CE1, CEH, CES1, Cocaine carboxylesterase, Egasyn, HMSE, HMSE1, Liver carboxylesterase 1, PCE 1, REH, Retinyl ester hydrolase, Serine esterase 1, SES1, TGH, Triacylglycerol hydrolase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CES1 Polyclonal antibody (16912-1-AP)

    WB analysis using 16912-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16912-1-AP (CES1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CES1 Polyclonal antibody (16912-1-AP)

    WB analysis of Jurkat using 16912-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16912-1-AP (CES1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CES1 Polyclonal antibody (16912-1-AP)

    IHC staining of human liver cancer using 16912-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16912-1-AP (CES1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CES1 Polyclonal antibody (16912-1-AP)

    IHC staining of human liver cancer using 16912-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16912-1-AP (CES1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CES1 Polyclonal antibody (16912-1-AP)

    IHC staining of human kidney using 16912-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16912-1-AP (CES1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CES1 Polyclonal antibody (16912-1-AP)

    IHC staining of human kidney using 16912-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16912-1-AP (CES1 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CES1 Polyclonal antibody (16912-1-AP)

    IHC staining of human liver cancer using 16912-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16912-1-AP (CES1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CES1 Polyclonal antibody (16912-1-AP)

    IHC staining of human liver cancer using 16912-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16912-1-AP (CES1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CES1 Polyclonal antibody (16912-1-AP)

    IF Staining of HepG2 using 16912-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16912-1-AP (CES1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16912-1-AP targets CES1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CES1 fusion protein Ag10567
Full Name carboxylesterase 1 (monocyte/macrophage serine esterase 1)
Calculated Molecular Weight 566 aa, 62 kDa
Observed Molecular Weight 60-63 kDa
GenBank Accession NumberBC012418
Gene Symbol CES1
Gene ID (NCBI) 1066
RRIDAB_2077500
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CES1(liver carboxylesterase 1) is also named as SES1 and belongs to the type-B carboxylesterase/lipase family. The deduced 567-amino acid protein contains a putative 18-amino acid signal peptide and a characteristic C-terminal endoplasmic reticulum retention signal (HXEL). It is the major hydrolytic enzyme responsible for the metabolism of numerous therapeutic agents as well as endogenous substrates.Westernblot analysis demonstrated that CES1 is expressed in human liver microsomes (HLM) but not in human intestinal microsomes (HIM)(PMID:19185566).

Protocols

Product Specific Protocols
WB protocol for CES1 antibody 16912-1-APDownload protocol
IHC protocol for CES1 antibody 16912-1-APDownload protocol
IF protocol for CES1 antibody 16912-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Brain

ATAD3 gene cluster deletions cause cerebellar dysfunction associated with altered mitochondrial DNA and cholesterol metabolism.

Authors - Radha Desai
View Article
humanWB,IHC

Clin Transl Med

Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.

Authors - Lijie Zhou
  • KO Validated
View Article
humanWB,IF

Oncotarget

Multifactorial resistance to aminopeptidase inhibitor prodrug CHR2863 in myeloid leukemia cells: down-regulation of carboxylesterase 1, drug sequestration in lipid droplets and pro-survival activation ERK/Akt/mTOR.

Authors - Sue Ellen Verbrugge
View Article
humanWB

Br J Pharmacol

PMID: 28128444

Authors - Wenjing Luo
View Article
humanWB

Drug Metab Dispos

Simultaneous absolute protein quantification of carboxylesterases 1 and 2 in human liver tissue fractions using liquid chromatography-tandem mass spectrometry.

Authors - Sato Yuichiro Y
View Article
mouseWB

J Pharm Pharmacol

Clock gene Bmal1 controls diurnal rhythms in expression and activity of intestinal carboxylesterase 1.

Authors - Xun Chen
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Maria (Verified Customer) (08-15-2021)

CES1 in human primary fibroblasts. 10 ug of protein. Blocked with 5% milk in PBST (0.1% Tween). 1:1000 primary ab O/N incubation at 4ºC. 1:5000 HRP conjugated secondary 2h RT.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human primary fibroblasts
CES1 Antibody Western Blot validation (1:1000 dilution) in Human primary fibroblasts (Cat no:16912-1-AP)