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  • Featured Product
  • KD/KO Validated

CDCA8 Polyclonal antibody

CDCA8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12465-1-AP

Synonyms

hDasra B, Dasra-B, Dasra B, Cell division cycle-associated protein 8, Borealin

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using CDCA8 Polyclonal antibody (12465-1-AP)

    WB analysis of mouse kidney using 12465-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12465-1-AP (CDCA8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using CDCA8 Polyclonal antibody (12465-1-AP)

    WB analysis of A549 using 12465-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12465-1-AP (CDCA8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CDCA8 Polyclonal antibody (12465-1-AP)

    IHC staining of human lung cancer using 12465-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12465-1-AP (CDCA8 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CDCA8 Polyclonal antibody (12465-1-AP)

    IHC staining of human lung cancer using 12465-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12465-1-AP (CDCA8 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDCA8 Polyclonal antibody (12465-1-AP)

    IF Staining of HeLa using 12465-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12465-1-AP (CDCA8 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, mouse kidney tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12465-1-AP targets CDCA8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDCA8 fusion protein Ag3136
Full Name cell division cycle associated 8
Calculated Molecular Weight 280 aa, 31 kDa
Observed Molecular Weight 31-38 kDa
GenBank Accession NumberBC016944
Gene Symbol CDCA8
Gene ID (NCBI) 55143
RRIDAB_2078783
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDCA8, also named as Borealin, Dasra-B and PESCRG3, is a component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. This protein can be migrated to 35-40 kDa with phosphor modification or ~50 kDa with SUMO modification.

Protocols

Product Specific Protocols
WB protocol for CDCA8 antibody 12465-1-APDownload protocol
IHC protocol for CDCA8 antibody 12465-1-APDownload protocol
IF protocol for CDCA8 antibody 12465-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Science

ESCRT-III governs the Aurora B-mediated abscission checkpoint through CHMP4C.

Authors - Jeremy G Carlton
View Article
humanIHC

Front Pharmacol

A novel risk model of three SUMOylation genes based on RNA expression for potential prognosis and treatment sensitivity prediction in kidney cancer

Authors - Song-Chao Li
View Article
humanWB,IHC

J Cell Mol Med

Cell division cycle associated 8: A novel diagnostic and prognostic biomarker for hepatocellular carcinoma

Authors - Xiao-Han Cui
View Article
mouseWB

Cell Mol Life Sci

Global phosphoproteomic analysis identified key kinases regulating male meiosis in mouse

Authors - Haojie Li
View Article
humanWB,IHC

Funct Integr Genomics

Let-7c-5p down-regulates immune-related CDCA8 to inhibit hepatocellular carcinoma

Authors - Wanjin Chen
View Article
humanIHC,WB

Am J Transl Res

Cell division cycle associated 8 promotes the growth and inhibits the apoptosis of endometrial cancer cells by regulating cell cycle and P53/Rb signaling pathway

Authors - Wenxue Li
  • KD Validated
View Article