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CDAN1 Polyclonal antibody

CDAN1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17597-1-AP

Synonyms

Codanin 1, codanin, CDAN 1, CDAI, CDA1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CDAN1 Polyclonal antibody (17597-1-AP)

    WB analysis of HeLa using 17597-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17597-1-AP (CDAN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using CDAN1 Polyclonal antibody (17597-1-AP)

    WB analysis of K-562 using 17597-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 17597-1-AP (CDAN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using CDAN1 Polyclonal antibody (17597-1-AP)

    IP experiment of K-562 using 17597-1-AP

    IP result of anti-CDAN1 (IP:17597-1-AP, 4ug; Detection:17597-1-AP 1:500) with K-562 cells lysate 3200ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDAN1 Polyclonal antibody (17597-1-AP)

    IF Staining of HeLa using 17597-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17597-1-AP (CDAN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, K-562 cells
Positive IP detected inK-562 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17597-1-AP targets CDAN1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDAN1 fusion protein Ag11589
Full Name congenital dyserythropoietic anemia, type I
Calculated Molecular Weight 1227 aa, 134 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC066640
Gene Symbol CDAN1
Gene ID (NCBI) 146059
RRIDAB_10694142
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDAN1 acts as a negative regulator of ASF1 in chromatin assembly. It appears to play a role in nuclear envelope integrity, possibly related to microtubule attachments. Mutations of CDAN1 cause congenital dyserythropoietic anemia type I, a disease resulting in morphological and functional abnormalities of erythropoiesis. There're some isoforms this gene.

Protocols

Product Specific Protocols
WB protocol for CDAN1 antibody 17597-1-APDownload protocol
IF protocol for CDAN1 antibody 17597-1-APDownload protocol
IP protocol for CDAN1 antibody 17597-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem J

A complex comprising C15ORF41 and Codanin-1: the products of two genes mutated in congenital dyserythropoietic anaemia type I (CDA-I).

Authors - Maithili Shroff
View Article