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CD7 Monoclonal antibody

CD7 Monoclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1G10D8

Cat no : 60209-1-Ig

Synonyms

T cell leukemia antigen, T cell antigen CD7, LEU-9, GP40, CD7 molecule

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CD7 Monoclonal antibody (60209-1-Ig)

    WB analysis of Jurkat using 60209-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60209-1-Ig (CD7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD7 Monoclonal antibody (60209-1-Ig)

    IHC staining of human appendicitis using 60209-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60209-1-Ig (CD7 antibody) at dilution of 1:3200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using CD7 Monoclonal antibody (60209-1-Ig)

    IHC staining of human appendicitis using 60209-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60209-1-Ig (CD7 antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD7 Monoclonal antibody (60209-1-Ig)

    IHC staining of human tonsillitis using 60209-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-1-Ig (CD7 antibody) at dilution of 1:1600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD7 Monoclonal antibody (60209-1-Ig)

    IHC staining of human tonsillitis using 60209-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-1-Ig (CD7 antibody) at dilution of 1:1600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD7 Monoclonal antibody (60209-1-Ig)

    IHC staining of human lymphoma using 60209-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 60209-1-Ig(CD7 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CD7 Monoclonal antibody (60209-1-Ig)

    IHC staining of human lymphoma using 60209-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 60209-1-Ig(CD7 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD7 Monoclonal antibody (60209-1-Ig)

    IF Staining of human tonsillitis using 60209-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60209-1-Ig (CD7 antibody), at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD7 Monoclonal antibody (60209-1-Ig)

    IF Staining of human tonsillitis using 60209-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60209-1-Ig (CD7 antibody), at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive IHC detected inhuman appendicitis tissue, human lymphoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1600-1:6400
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60209-1-Ig targets CD7 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD7 fusion protein Ag1805
Full Name CD7 molecule
Calculated Molecular Weight 240 aa, 25 kDa
GenBank Accession NumberBC009293
Gene Symbol CD7
Gene ID (NCBI) 924
RRIDAB_10953398
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD7 is a 40 kD type I transmembrane glycoprotein expressed on thymocytes and mature T cells. It is the most reliable clinical marker of T-cell acute lymphocytic leukemia.

Protocols

Product Specific Protocols
WB protocol for CD7 antibody 60209-1-IgDownload protocol
IHC protocol for CD7 antibody 60209-1-IgDownload protocol
IF protocol for CD7 antibody 60209-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF,FC

Cell Metab

Bone marrow immune cells respond to fluctuating nutritional stress to constrain weight regain

Authors - Hai-Yan Zhou
View Article

Small

In Vivo mRNA CAR T Cell Engineering via Targeted Ionizable Lipid Nanoparticles with Extrahepatic Tropism

Authors - Margaret M Billingsley
View Article