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CD7 Polyclonal antibody

CD7 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 11320-1-AP

Synonyms

T cell surface antigen Leu-9, T cell leukemia antigen, T cell antigen CD7, LEU-9, GP40

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD7 Polyclonal antibody (11320-1-AP)

    IHC staining of human tonsillitis using 11320-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11320-1-AP (CD7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD7 Polyclonal antibody (11320-1-AP)

    IHC staining of human tonsillitis using 11320-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11320-1-AP (CD7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD7 Polyclonal antibody (11320-1-AP)

    IHC staining of human lung cancer using 11320-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11320-1-AP (CD7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD7 Polyclonal antibody (11320-1-AP)

    IHC staining of human lung cancer using 11320-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11320-1-AP (CD7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD7 Polyclonal antibody (11320-1-AP)

    IF Staining of human tonsillitis using 11320-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD7 antibody (11320-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD7 Polyclonal antibody (11320-1-AP)

    IF Staining of human tonsillitis using 11320-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD7 antibody (11320-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inhuman tonsillitis tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11320-1-AP targets CD7 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD7 fusion protein Ag0486
Full Name CD7 molecule
Calculated Molecular Weight 240 aa, 25 kDa
GenBank Accession NumberBC009293
Gene Symbol CD7
Gene ID (NCBI) 924
RRIDAB_2877758
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD7 is a 40 kD type I transmembrane glycoprotein expressed on thymocytes and mature T cells. It is the most reliable clinical marker of T-cell acute lymphocytic leukemia.

Protocols

Product Specific Protocols
IHC protocol for CD7 antibody 11320-1-APDownload protocol
IF protocol for CD7 antibody 11320-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols